| 一株高浓度烟碱降解菌的筛选、分离和初步鉴定 |
| |
| 引用本文： | 龙章德,韦方立,李俊芳,田兆福,张敏,黎新钦,申佩弘.一株高浓度烟碱降解菌的筛选、分离和初步鉴定[J].广西农业生物科学,2013(5):612-615. |
| |
| 作者姓名： | 龙章德 韦方立 李俊芳 田兆福 张敏 黎新钦 申佩弘 |
| |
| 作者单位： | [1]广西中烟工业有限责任公司,南宁530001 [2]广西民族师范学院化学与生物工程系,崇左532200 [3]广西大学生命科学与技术学院,南宁530004 |
| |
| 基金项目： | 基金项目：本研究由广西中烟工业有限责任公司创新项目（20100037）资助 |
| |
| 摘 要： | 采用以烟碱为唯一碳源氮源的选择培养基，从烟草和植烟土壤中分离筛选出15株高浓度烟碱降解的菌株，其中菌株D9烟碱降解能力最强，其烟碱降解率为82％，耐受烟碱的最高浓度为8g／L。经常规形态特征、16SrDNA同源序列以及系统进化树分析，初步鉴定该菌株为类似氧化微杆菌，命名为Microbacteriumsp．GYC29。本研究为微生物降解烟碱的研究及应用提供了菌种资源。
|
| 关 键 词： | 烟碱 生物降解 烟叶 微杆菌 |
Screening,Isolation and Identification of a High Concentration Nicotine Degradation Bacterium Strain |
| |
| Long Zhangde Wei Fangli Li Junfang Tian Zhaofu,Zhang Min,Li Xinqin Shen Peihong.Screening,Isolation and Identification of a High Concentration Nicotine Degradation Bacterium Strain[J].Journal of Guangxi Agricultural and Biological Science,2013(5):612-615. |
| |
| Authors: | Long Zhangde Wei Fangli Li Junfang Tian Zhaofu Zhang Min Li Xinqin Shen Peihong |
| |
| Institution: | Long Zhangde Wei Fangli Li Junfang Tian Zhaofu Zhang Min Li Xinqin Shen Peihong 1 China Tobacco Guangxi Industrial Co., Ltd., Nanning, 530001; 2 Chemical and Biological Engineering Guangxi Normal University for Nationali- ties, Chongzuo, 532200; 3 College of Life Science and Technology, Guangxi University, Nanning, 530005 |
| |
| Abstract: | 15 nicotine-degrading bacterial strains were separated and screened from tobacco and soil with selectivity culture medium, strain D9 was the best one with the highest nicotine degradation ratio reached at 82%, and could grow in a high nicotine concentration reached at 8 g/L using nicotine as the sole carbon and nitrogen source. The strain D9 was identified as Microbacterium sp. GYC29 according to the normal morphology, physiological and bio- chemical characters and 16S rDNA gene sequence. |
| |
| Keywords: | Nicotine Biodegradation Tobacco Microbacterium |
| 本文献已被 维普 等数据库收录！ |
