| 核桃BES-SSR的开发及在遗传多样性分析中的应用 |
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| 引用本文： | 陈凌娜,马庆国,张俊佩,周贝贝,裴东.核桃BES-SSR的开发及在遗传多样性分析中的应用[J].北京林业大学学报,2014,36(6):24-29. |
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| 作者姓名： | 陈凌娜 马庆国 张俊佩 周贝贝 裴东 |
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| 作者单位： | 北京林业大学生物科学与技术学院;中国林业科学研究院林业研究所，林木遗传育种国家重点实验室;中国林业科学研究院林业研究所，林木遗传育种国家重点实验室 |
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| 基金项目： | 国家自然科学基金项目(31171933)。 |
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| 摘 要： | 从NCBI 数据库全部已知核桃MboI 基因组BAC 文库克隆中下载22 740 条末端序列(BAC end sequences,
BES)。应用MISA 软件搜索获得SSR 位点4 732 个,平均每2.8 kb 出现1 个SSR。在含有单个SSR 的BES 中,1 ~3
个核苷酸重复的类型占SSR 总数的96.86%,A/ T、AT/ AT、ATT/ AAT 分别为最丰富的单核苷酸、2 核苷酸和3 核苷
酸重复。选择不同类型和重复次数的SSR 设计合成50 对引物,从中筛选出多态性引物33 对,其中19 对引物扩增
出1 或2 个等位基因,无非特异扩增产物。应用这19 对引物对20 份核桃属不同种的基因型进行SSR 分析,结果表
明,每对引物检测到2 ~9 个多态性位点,平均多态性位点5.4 个;其中17 个位点的多态信息含量高于0.5,总平均
值为0.662,多态性较高。聚类分析显示,不同基因型按照种属分类及地理起源分组。因此,BES-SSR 是一类多态
性高,可用于核桃属植物遗传分析的新SSR 引物。
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| 关 键 词： | 核桃 BAC末端序列 SSR 遗传多样性 |
Development of BAC-SSR markers in walnut and its application in genetic diversity analysis |
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| CHEN Ling-na,MA Qing-guo,ZHANG Jun-pei,ZHOU Bei-bei,PEI Dong.Development of BAC-SSR markers in walnut and its application in genetic diversity analysis[J].Journal of Beijing Forestry University,2014,36(6):24-29. |
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| Authors: | CHEN Ling-na MA Qing-guo ZHANG Jun-pei ZHOU Bei-bei PEI Dong |
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| Institution: | 1 College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, P. R. China; |
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| Abstract: | From all known Juglans regia MboI genomic BAC clones in NCBI database, 22 740 end sequences
(BES) were downloaded and analyzed. A total of 4 732 SSRs (microsatellites) were found by using the
MISA program. The frequency of SSRs was approximately 1/2.8 kb. Mono-to tri-nucleotide types
accounted for 96. 86% of all the single SSRs in the BES. A/ T, AT/ AT and ATT/ AAT motifs were the
repeat categories of most abundant mono-, di-and tri-nucleotide respectively. Fifty SSR primer pairs with
different types and number of repeats were designed and synthesized, and 33 highly polymorphic SSR
primer pairs were screened. Nineteen out of the 33 primer pairs had 1 or 2 alleles without nonspecific
amplification. Those primer pairs were used to amplify SSR for 20 genotypes in the genus Juglans. The
results showed that 2 - 9 polymorphic loci, 5.4 on average, were amplified per pair of primers.
Polymorphism information content was greater than 0.5 in 17 SSRs loci with a mean value of 0.662 in all
loci, which showed high polymorphism. The cluster analysis showed that the groups were clustered in
accordance with species and origin. Our findings indicated that BES-SSRs identified in J. regia had high
polymorphism and were new SSR primers for genetic analysis of Juglans. |
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| Keywords: | Juglans regia BAC end sequence SSR genetic diversity |
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