| 低温胁迫对赤霉素代谢的调控研究 |
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| 引用本文： | 高琼,钮世辉,李伟,陈晓阳.低温胁迫对赤霉素代谢的调控研究[J].北京林业大学学报,2014,36(6):135-141. |
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| 作者姓名： | 高琼 钮世辉 李伟 陈晓阳 |
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| 作者单位： | 北京林业大学生物科学与技术学院，林木育种国家工程实验室;北京林业大学生物科学与技术学院，林木育种国家工程实验室;华南农业大学林学院 |
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| 基金项目： | 中央高校基本科研业务费专项资金(YX2014-12)。 |
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| 摘 要： | 通过4℃处理,对模式植物拟南芥与烟草赤霉素(GA)代谢相关基因的表达进行了分析,结果表明:低温胁迫
抑制赤霉素前体GGPP 合成通路众多基因的表达,而促进催化GA 失活酶编码基因及赤霉素受体基因的表达。通
过对超表达PtGA20ox、PtGA2ox1 及AtCBF1 的转基因烟草及4℃处理野生型烟草植株GA1 含量及代谢相关基因的
表达分析表明,低温胁迫下,GGPP 合成与GA 失活酶编码基因的表达属于主动调控,低温响应通路中关键因子
CBF1 可能参与了此调控过程,而赤霉素受体基因表达受到了低水平GA 的反馈调控。该研究揭示低温胁迫下生长
延滞可能是植物主动响应环境变化的过程,通过抑制前体积累及加速失活而降低GA 水平可能在植物适应低温胁
迫过程中发挥着重要作用。
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| 关 键 词： | 赤霉素 低温胁迫 CBF1 GGPP |
Regulation of low temperature stress on gibberellin metabolism |
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| GAO Qiong,NIU Shi-hui,LI Wei,CHEN Xiao-yang.Regulation of low temperature stress on gibberellin metabolism[J].Journal of Beijing Forestry University,2014,36(6):135-141. |
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| Authors: | GAO Qiong NIU Shi-hui LI Wei CHEN Xiao-yang |
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| Institution: | 1 National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, P. R. China; |
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| Abstract: | The expression of genes involved in gibberellin ( GA) metabolism in model plants Arabidopsis
thaliana and tobacco (Nicotiana tabacum) under the 4℃treatment was analyzed. The results indicated
that the expressions of many genes in geranylgeranyl diphosphate ( GGPP) synthesis pathway were
inhibited, while the expressions of GA deactivation genes and GA receptor genes were promoted. Analysis
of GA1 content and the expression metabolism-related genes in over-expressed PtGA20ox, PtGA2ox1 and
AtCBF1 transgenic and 4℃-treated wild type tobacco plants showed that the expression of GGPP
synthesis genes and GA deactivation genes was active regulation, and the key player, CBF1 in cold
response pathway, participated in this regulation process, while the expression of gibberellin receptor
genes was feedback regulation by the low levels of GA. This study revealed that growth retardation of
plants under cold stress may result from their positive response to the environment. Decreasing GA level
by limiting the supply of precursor accumulation and accelerating the deactivation may play a vital role in
the process of plants adapting to the cold stress. |
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| Keywords: | gibberellin cold stress CBF1 GGPP |
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