| 斜纹夜蛾P450基因CYP4M14和CYP4S9的克隆与mRNA表达水平研究 |
| |
| 引用本文： | 黄水金,秦文婧,陈琼.斜纹夜蛾P450基因CYP4M14和CYP4S9的克隆与mRNA表达水平研究[J].中国农业科学,2010,43(15):3115-3124. |
| |
| 作者姓名： | 黄水金 秦文婧 陈琼 |
| |
| 作者单位： | (江西省农业科学院植物保护研究所) |
| |
| 摘 要： | 【目的】研究斜纹夜蛾对溴氰菊酯抗性的分子机制。【方法】利用RT-PCR和RACE技术克隆CYP4M14和CYP4S9 cDNA全长序列,采用实时定量PCR技术分析这两个基因在斜纹夜蛾抗、感品系6龄幼虫中肠和脂肪体中mRNA 的表达水平。【结果】克隆得到斜纹夜蛾细胞色素P450基因CYP4M14和CYP4S9的cDNA全长序列,序列分析表明,CYP4M14编码区长1 512 bp,编码 504个氨基酸;CYP4S9编码区长1 473 bp,编码 491个氨基酸;实时定量PCR分析结果表明,在中肠中,CYP4M14和CYP4S9在抗性品系中的mRNA表达量分别是敏感品系中的4.85和18.63倍;在脂肪体中分别是在敏感品系中的75.14和6.07倍。【结论】斜纹夜蛾对溴氰菊酯的高水平抗性可能与CYP4M14和CYP4S9的过量表达有关。
|
| 关 键 词： | 斜纹夜蛾 细胞色素P450 实时定量PCR P450基因 |
| 收稿时间： | 2009-12-28; |
Cloning and mRNA Expression Levels of Cytochrome P450 Genes CYP4M14 and CYP4S9 in the Common Cutworm Spodoptera litura(Fabricius) |
| |
| HUANG Shui-jin,QIN Wen-jing,CHEN Qiong.Cloning and mRNA Expression Levels of Cytochrome P450 Genes CYP4M14 and CYP4S9 in the Common Cutworm Spodoptera litura(Fabricius)[J].Scientia Agricultura Sinica,2010,43(15):3115-3124. |
| |
| Authors: | HUANG Shui-jin QIN Wen-jing CHEN Qiong |
| |
| Institution: | (Institute of Plant Protection, Jiangxi Academy of Agricultural Sciences)
|
| |
| Abstract: | 【Objective】 Molecular mechanism of deltamethrin resistance in the common cutworm was studied. 【Method】Two novel P450 genes (CYP4M14 and CYP4S9) of Spodoptera litura were cloned using the RT-PCR with degenerate primers and rapid amplification of cDNA ends (RACE) strategies, and mRNA expression levels of CYP4M14 and CYP4S9 were compared between the resistant and susceptible strain, respectively. 【Result】 The coding region of CYP4M14 cDNA was 1 512 bp, encoding a 504-amino-acid protein, while that of CYP4S9 cDNA was 1 473 bp, encoding a 491-amino-acid protein. Relative mRNA expression levels of CYP4M14 and CYP4S9 in the resistant strain were compared with that in the susceptible strain using real-time quantitative PCR. It was found that CYP4M14 and CYP4S9 in the resistant strain were expressed 4.85 and 18.63 fold higher in the midgut and 75.14 and 6.07 fold in the fatbody, respectively. 【Conclusion】 Constitutive overexpression of CYP4M14 and CYP4S9 may likely contribute to deltamethrin resistance in S. litura. |
| |
| Keywords: | Spodoptera litura cytochrome P450 real-time quantitative PCR P450 genes |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
