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水杨酸和乙烯在烟草抗黑胫病中的作用
引用本文:王长春,蔡新忠,林敬州,郑重.水杨酸和乙烯在烟草抗黑胫病中的作用[J].植物保护学报,2003,30(3):295-299.
作者姓名:王长春  蔡新忠  林敬州  郑重
作者单位:浙江大学植物保护系,杭州,310029
摘    要:5mmol/L水杨酸或1g/L乙烯外源处理可诱导烟草对黑胫病的抗性,推迟植株死亡。不能积累水杨酸的转nahG基因植株和不表现乙烯应答反应的转etr1-1基因植株对黑胫病的抗性显著弱于野生型植株。组成性积累高水平水杨酸的转entC和pmsB基因植株对黑胫病的抗性明显强于野生型植株。这些结果说明水杨酸和乙烯增强烟草对黑胫病的抗性,减缓黑胫病的扩展。0.5mmol/L KCN连续浇灌植株4天或浸溃叶片2,4h均不能恢复转nahG基因植株丧失的对黑胫病的抗病性。此结果进一步支持关于KCN处理不能恢复转nahG基因植株丧失的对真菌病害抗病性的假说。

关 键 词:水杨酸  乙烯  烟草  抗黑胫病  抗性
收稿时间:2002/12/23 0:00:00
修稿时间:2002年12月23

The role of salicylic acid and ethylene in resistance of tobacco to black shank
Wang Changchun,Cai Xinzhong,Lin Jingzhou and Zheng Zhong.The role of salicylic acid and ethylene in resistance of tobacco to black shank[J].Acta Phytophylacica Sinica,2003,30(3):295-299.
Authors:Wang Changchun  Cai Xinzhong  Lin Jingzhou and Zheng Zhong
Institution:Department of Plant Protection, Zhejiang University, Hangzhou 310029, China,Department of Plant Protection, Zhejiang University, Hangzhou 310029, China,Department of Plant Protection, Zhejiang University, Hangzhou 310029, China and Department of Plant Protection, Zhejiang University, Hangzhou 310029, China
Abstract:Treatment of tobacco leaf with 5 mmol/L salicylic acid (SA) or 1 g/L ethylene induced resistance to black shank and delayed the death of infected plants. Resistance of plants transformed with nahG, which was unable to accumulate SA, or etrI-1 , which does not respond to ethylene, was significantly weaker than that of wild type plants. Resistance of plants transformed with entC and pmsB, which constitutively accumulate SA to a high level, was stronger than that of wild type plants. These results revealed that SA and ethylene enhanced resistance of tobacco to black shank and attenuated the spread of the pathogen. Treatments of continuous watering plant for 4d or soaking leaf for 24h with KCN did not restore the resistance lost in nahG transgenic plant. This result supported the hypothesis that KCN was not able to restore the resistance to fungal diseases lost in nahG transgenic plant.
Keywords:salicylic acid  ethylene  tobacco black shank  resistance  
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