| 利用菌落PCR-DGGE快速鉴定盐渍土壤菌种多样性 |
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| 引用本文： | 张海燕,贺江舟,龚明福,刘占文,张利莉.利用菌落PCR-DGGE快速鉴定盐渍土壤菌种多样性[J].塔里木农垦大学学报,2007,19(4):30-32. |
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| 作者姓名： | 张海燕 贺江舟 龚明福 刘占文 张利莉 |
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| 作者单位： | 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆,阿拉尔,843300 |
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| 基金项目： | 塔里木农垦大学校科研和教改项目;新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室开发课题;塔里木大学校科研和教改项目 |
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| 摘 要： | 利用菌落PCR方法,结合DGGE的检测方法,对所保存的87份盐生菌的培养物进行了多样性检测,初步研究显示至少有31株不同的菌种,表明新疆盐生环境存在较为丰富的菌种资源,同时表明菌落PCR结合DGGE技术为细菌鉴定和菌种保藏过程中重复菌株的去除提供了一种快速可靠的手段。
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| 关 键 词： | 菌落PCR 多样性 菌种保存 |
| 文章编号： | 1009-0568（2007）04-0030-03 |
| 收稿时间： | 2007-07-20 |
| 修稿时间： | 2007年7月20日 |
A Method Based on Bacterial Colony PCR-DGGE for Rapid Determination of Microbial Diversity in Xinjiang Salt-soil |
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| Zhang Haiyan,He Jiangzhou,Gong Mingfu,Liu Zhanwen,Zhang Lili.A Method Based on Bacterial Colony PCR-DGGE for Rapid Determination of Microbial Diversity in Xinjiang Salt-soil[J].Journal of Tarim University of Agricultural Reclamation,2007,19(4):30-32. |
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| Authors: | Zhang Haiyan He Jiangzhou Gong Mingfu Liu Zhanwen Zhang Lili |
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| Abstract: | In this paper,the combination of bacterial colony PCR and denaturing gradient electrophoresis were used in the microbial diversity analysis of 87 halo inhabited bacterial culture collections.The results showed the recovered 87 culture collections contain at least 31 different strains,which confirmed that the combination of colony PCR and DGGE is a reliable and time-conserved approach to eliminate the repetitive strains in bacterial identification and strain conservation.The results showed there was great microbial diversity in salt-soil in Xinjiang. |
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| Keywords: | GGE |
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