| 利用SSR技术快速准确鉴定杂交玉米种子纯度 |
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| 引用本文： | 吴明生,贾希海,田雷,律宝春.利用SSR技术快速准确鉴定杂交玉米种子纯度[J].分子植物育种,2006,4(3):381-384. |
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| 作者姓名： | 吴明生 贾希海 田雷 律宝春 |
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| 作者单位： | 北京市种子管理站,北京,100088 |
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| 摘 要： | 利用一种快速、廉价的DNA提取方法,提取了两个玉米杂交种及其亲本的DNA用于SSR分子标记分析。分析结果显示,10对被分析的引物中有4对在两个杂交种双亲之间显示出多态性,可以用于相应的杂交种纯度分析。另外,分别利用同工酶技术和SSR分子标记技术同时分析了来自这两个杂交种的种子样品的纯度,分析发现,对于一个杂交种,两种方法都能可以鉴定,并且两者检测的结果是一致的,但是对于另外一个杂交种,由于其父母本非常接近,同工酶不能将其区分。因此,这些结果显示SSR分子标记技术可以很好地用于杂交玉米种子的纯度鉴定,即使是这个杂交种是来自两个非常接近的自交系的。
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| 关 键 词： | 玉米 SSR标记 纯度鉴定 F1杂交种 |
Rapid and Reliable Purity Identification of F1 Hybrids of Maize (Zea may L.) Using SSR Markers |
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| Wu Mingsheng,Jia Xihai,Tian Lei,Lv Baochun.Rapid and Reliable Purity Identification of F1 Hybrids of Maize (Zea may L.) Using SSR Markers[J].Molecular Plant Breeding,2006,4(3):381-384. |
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| Authors: | Wu Mingsheng Jia Xihai Tian Lei Lv Baochun |
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| Abstract: | Using a rapid and cheap DNA extraction method, genomic DNA extracted from two maize F1 hybrids and their parental lines was subjected to SSR analysis with 10 pairs of primer. Four of them could detect polymorphism between the male parents and the female parents of the two hybrids, which could be used for purity assessment of the hybrids. Moreover, the purity of seed samples of the two F1 hybrids was identified using the SSR analysis with the four pairs of primer and isozyme analysis respectively. For one hybrid, the results obtained using the two methods are consistent, but for the other one, isozyme analysis failed to detect any polymorphism between the parental lines because they are closely related. These results clearly demonstrate that SSR marker should be useful for assessing purity of maize hybrid, even if the hybrid derived from two related parental lines. |
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| Keywords: | Maize(Zea may L ) SSR marker Purity identification F1 hybrid |
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