| 4个多用途萱草品种离体快繁体系的建立 |
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| 引用本文： | 储博彦,张全锋,高超利,李金霞,赵玉芬.4个多用途萱草品种离体快繁体系的建立[J].安徽农业科学,2014(14):4189-4191,4214. |
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| 作者姓名： | 储博彦 张全锋 高超利 李金霞 赵玉芬 |
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| 作者单位： | 河北省林业科学研究院;河北省林木良种工程技术研究中心;河北农业大学园林与旅游学院; |
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| 基金项目： | 石家庄市科技支撑计划课题“多用途萱草优良品种引选及栽培技术研究”(项目编号:11152052A) |
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| 摘 要： | 目的]建立4个多用途萱草品种的离体快繁体系.方法]以4个多用途萱草品种为试材,研究消毒时间对不同外植体的影响,以及不同激素配比的培养基对愈伤组织、增殖培养和生根培养的影响.结果]消毒时间以10 min最佳,花茎是萱草组织培养的最佳外植体;愈伤组织诱导的最佳培养基为：MS＋ 1.5 mg/L 6-BA ＋0.2 mg/L IBA;在增殖培养中,MS＋ 1.5 mg/L 6-BA＋ 0.2 mg/L IBA为最佳增殖培养基.1/2MS ＋0.3 mg/L IBA和1/2MS＋ 0.3 mg/L NAA培养基为4个品种的最佳生根培养基.结论]该方法建立了4个多用途萱草品种的离体快繁体系,为萱草的种质资源栽培提供了理论依据.
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| 关 键 词： | 多用途萱草 组织培养 培养基 外植体 快繁体系 |
Studies on Tissue Culture and Rapid Propagation of the Four Multi-role Hemerocallis fulva |
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| Institution: | CHU Bo-yan et al( Hebei Institute of Forestry Science, Shijiazhuang, Hebei 050061) |
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| Abstract: | Objective] To establish tissue culture and rapid propagation of the four multi-role Hemerocallis fulva.Method] With the four multi-role H.fulva as test materials,effects of disinfection time on different explants were studied,effects of culture medium with different hormone ration on tissue callus,proliferation and rooting culture were studied.Result] The results showed that 10 min was the best disinfection time,and the best explant was scape; The best medium for callus was MS ＋ 1.5 mg/L6-BA ＋ 0.2 mg/L IBA ; Shoot reproduction medium was MS ＋ 1.5 mg/L6-BA ＋ 0.2 mg/L IBA ; The best rooting medium was 1/2MS ＋ 0.3 mg/L IBA and 1/2MS ＋ 0.3 mg/L NAA.Conclusion] The tissue cuhure and rapid propagation of four multi-role Hemerocallis fulva were established,which will provide theoretical basis for germplasm resource cultivation of Hemerocallis fulva. |
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| Keywords: | Multi-role Hemerocallis fulva Tissue culture Culture medium Explants Rapid propagation |
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