| 改良CTAB法提取冷季型草坪草基因组DNA的研究 |
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| 引用本文： | 杨亚亚,麻冬梅,许兴.改良CTAB法提取冷季型草坪草基因组DNA的研究[J].北方园艺,2009(5). |
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| 作者姓名： | 杨亚亚 麻冬梅 许兴 |
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| 作者单位： | 宁夏大学,生命科学学院,宁夏,银川,750021;宁夏大学农学院,宁复,银川,750021;宁夏大学,生命科学学院,宁夏,银川,750021;宁夏大学农学院,宁复,银川,750021 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 为获得高质量的冷季型草坪草基因组DNA,用改良和优化常规CTAB提取方法,提取冷季型草坪草基因组DNA,并对所得DNA的质量和浓度进行了检测.结果表明:改良CTAB-B法要好于常规CTAB法和改良CTAB-A法,提取的基因组DNA的OD260/OD280在1.8～1.9之间,电泳条带清晰,RNA消化彻底,DNA无明显降解,其含量和纯度均能满足基因工程操作的要求,且酶切效果理想.
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| 关 键 词： | 基因组DNA 改良CTAB法 DNA提取 酶切分析 |
Studies on Genomic DNA Extraction of Cool-season Turf Grass with Improved CTAB Method |
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| YANG Ya-ya,MA Dong-mei,XU Xing.Studies on Genomic DNA Extraction of Cool-season Turf Grass with Improved CTAB Method[J].Northern Horticulture,2009(5). |
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| Authors: | YANG Ya-ya MA Dong-mei XU Xing |
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| Institution: | 1.Life Sciences Academy;Ningxia University;Yinchuan;Ningxia 750021;China;2.Agricultural College;China |
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| Abstract: | Genome DNA was extracted by the improved CTAB method from Cool-season turf grass and the quality and concentration of DNA were detected.The results indicated that improved CTAB-B method was a simple and high efficient method which could obtain the high quality of genome DNA,the value of OD_260/OD_280 was 1.8 to 1.9,the RNA was eliminated completely without obvious degradation of DNA,the content and purity can meet the requirements of genetic engineering,and digested the results were satisfactory. |
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| Keywords: | Genomic DNA Improved CTAB method DNA extraction Analysis of enzymatic digestion |
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