| 茄子组织培养与基因工程研究进展 |
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| 引用本文： | 金丹丹,梁美霞,谢立波,李景富.茄子组织培养与基因工程研究进展[J].分子植物育种,2004,2(6):861-866. |
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| 作者姓名： | 金丹丹 梁美霞 谢立波 李景富 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
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| 摘 要： | 本文综述了茄子组织培养与基因工程技术所取得的进展，并对茄子基因工程的发展进行了展望。迄今已利用茄子幼苗子叶、胚轴、叶片、胚、茎等外植体获得再生植株，是茄子离体再生研究最多的一个领域。其再生的关键取决于材料基因型、外植体类型、培养基类型等因素，不同基因型材料的再生能力差异很大，也影响再生的途径。原生质体是比较理想的进行遗传操作的受体，所以在其来源、培养液组成和成苗途径方面都有研究。花药和小孢子离体培养可以获得单倍体植株，节省获得纯系的时间，并有利于获得重组家系，为生产商用F1杂种开辟了一条重要途径。通过幼苗外植体器官再生、花粉(花药)培养、原生质体和小孢子培养已成功地建立了茄子受体再生系统。茄子基因工程技术在提高现有品质和创新种质方面发挥了积极的作用，农杆菌介化法是茄子转基因常用方法，已在外植体选择及再生、选择压筛选、农杆菌株系和转化浓度、预培养和共培养时间等影响因子方面做了大量的优化工作。转基因技术主要应用于茄子抗虫和单性结实基因工程方面并分别获得了一系列成果。在其他领域如抗病、抗逆等方面应用还很不足。因此作者认为未来的研究工作应集中于：目的基因的标记与克隆、高效受体再生系统的探索、更有效遗传转化方法的应用、扩大遗传转化范围，以提高实际应用水平。
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| 关 键 词： | 茄子 组织培养 基因工程 离体再生 农杆菌介化法 转基因 |
Advances in Eggplant Tissue Culture and Genetic Engineering |
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| Jin Dandan Liang Meixia Xie Libo Li JingfuHorticulture College,Northeast Agricultural University,Harbin.Advances in Eggplant Tissue Culture and Genetic Engineering[J].Molecular Plant Breeding,2004,2(6):861-866. |
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| Authors: | Jin Dandan Liang Meixia Xie Libo Li JingfuHorticulture College Northeast Agricultural University Harbin |
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| Institution: | Jin Dandan Liang Meixia Xie Libo Li Jingfu*Horticulture College,Northeast Agricultural University,Harbin,150030 |
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| Abstract: | This paper summarized the development of tissue culture and genetic engineering of eggplant, and also discussed the prospect on application of genetic engineering in eggplant breeding. The most studied regeneration system has been established through the culture of cotyledon?hypocotyl?leave?embryo and stalk. The regeneration is influenced by plant genotype?different explants?type of medium etc. Different genotype has different regeneration ability and approach. Because of its excellence as the object of genetic transformation, protoplasts has been studied a lot on its sources?component of the culture liquid and growing method. Haploid acquired through the culture of pollen and microspore can been used by the production of F1 hybrid. So far, the regeneration system has been established through the culture of seeding explants?pollens?protoplasts and microspores. Genetic engineering of eggplant plays an important role in enhancement of existing quality and creation of new germplasm. The Agrobacterium-mediated method is the usual way in eggplant genetic engineering, and has been optimized in the select and regeneration of the explants?select of the antibiotic concentration and bacterium concentration?days of advance culture and corporate culture. Genetic engineering of eggplant is mainly used in insect resistance and parthenocarpy, but it is rarely used in other field such as resistance of disease and adversity. So, the author think that the future research should center on: the mark and clone of the destination gene?the explore of efficient regeneration system?the application of transgene methods and scale the use of it. |
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| Keywords: | Eggplant Tissue culture Genetic engineering |
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