| 不同DNA提取方法对隐孢子虫卵囊PCR检测的影响 |
| |
| 引用本文： | 陈兆国,米荣升,周鹏,黄燕,张国恩,苏庆美,秦培兰,呼高伟,林矫矫.不同DNA提取方法对隐孢子虫卵囊PCR检测的影响[J].中国兽医寄生虫病,2011,19(1):57-61. |
| |
| 作者姓名： | 陈兆国 米荣升 周鹏 黄燕 张国恩 苏庆美 秦培兰 呼高伟 林矫矫 |
| |
| 作者单位： | 中国农业科学院上海兽医研究所,农业部动物寄生虫学重点开放实验室,中国农业科学院动物源性食品安全研究中心,上海,200241 |
| |
| 基金项目： | 上海市科技兴农重点攻关项目，国家"十一五"高技术研究发展计划863项目，国家"十一五"科技支撑计划项目 |
| |
| 摘 要： | 为了提高隐孢子虫PCR检测的敏感性和效率,采用10种基因组DNA提取方法,对隐孢子虫卵囊DNA进行提取,对提取的DNA进行nested PCR扩增。经过3次重复试验,结果显示,Chelex 100法、FTA试纸法和Wizard DNAClean-Up System试剂盒法敏感性最高,能够稳定地扩增出1×10^2个卵囊提取的DNA,适合隐孢子虫病分子流行病学调查时大量样品DNA的提取。
|
| 关 键 词： | 隐孢子虫 DNA 提取 |
INFLUENCE OF DIFFERENT DNA EXTRACTION METHODS OF CRYPTOSPORIDIUM OOCYSTS ON PCR DETECTION |
| |
| CHEN Zhao-guo,MI Rong-sheng,ZHOU Peng,HUANG Yan,ZHANG Guo-en,SU Qing-mei,QIN Pei-lan,HU Gao-wei,LIN Jiao-jiao.INFLUENCE OF DIFFERENT DNA EXTRACTION METHODS OF CRYPTOSPORIDIUM OOCYSTS ON PCR DETECTION[J].Chinese Journal of Veterinary Parasitology,2011,19(1):57-61. |
| |
| Authors: | CHEN Zhao-guo MI Rong-sheng ZHOU Peng HUANG Yan ZHANG Guo-en SU Qing-mei QIN Pei-lan HU Gao-wei LIN Jiao-jiao |
| |
| Institution: | CHEN Zhao-guo,MI Rong-sheng,ZHOU Peng,HUANG Yan,ZHANG Guo-en,SU Qing-mei,QIN Pei-lan,HU Gao-wei,LIN Jiao-jiao (Animal-borne Food Safety Research Center of Chinese Academy of Agricultural Sciences,Key Laboratory of Animal Parasitology of the Ministry of Agriculture,Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China) |
| |
| Abstract: | In order to improve the sensitivity and efficiency of PCR detection of Cryptosporidium,ten kinds of methods were used to extract genomic DNA of Cryptosporidium oocysts.The extracted DNAs were used as template to amplify a small subunit ribosomal RNA（SSU rRNA）fragment with Nested PCR.The results of 3 times duplicate test showed that Chelex 100,FTA filter paper,Wizard DNA Clean-Up System were the three most sensitive methods which could extract genomic DNA of 1×102 Cryptosporidium oocysts for nested PCR amplification and can be used in large number of detections for cryptosporidiosis molecular epidemiology research. |
| |
| Keywords: | Cryptosporidium DNA extraction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
