|
|||||
|
|
| 喹乙醇诱导鲤肝细胞凋亡的研究 | |
| 引用本文： | 汪开毓.喹乙醇诱导鲤肝细胞凋亡的研究[J].水产学报,2004,28(6):733-737. |
| 作者姓名： | 汪开毓 |
| 作者单位： | 1. 四川农业大学水产系,四川,雅安,625014 2. 中国农业大学动物医学院,北京,100094 3. 四川省水产研究所,四川,成都,617731 |
| 基金项目： | 四川省学术带头人后备人选资助基金 |
| 摘 要： | 细胞凋亡(apoptosis)是受基因控制的细胞自主的有序死亡。凋亡细胞的形态特征主要表现为细胞核的染色质浓缩,边移,聚集在核膜下,进而核发生裂解,核碎片与细胞碎片有单层膜包裹形成凋亡小体(apoptosisbody)1]。目前已发现某些物理因素(辐射、高温等)、细胞因子、病毒感染和 |
| 关 键 词： | 鲤 细胞凋亡 喹乙醇 肝细胞 |
| 文章编号： | 1000-0615(2004)06-0733-05 |
| 收稿时间： | 2014/3/14 0:00:00 |
| 修稿时间： | 2003年10月27 |
Study on hepatic cells apoptosis induced by olaquindox in Cyprinus carpio |
|
| WANG Kai-yu.Study on hepatic cells apoptosis induced by olaquindox in Cyprinus carpio[J].Journal of Fisheries of China,2004,28(6):733-737. | |
| Authors: | WANG Kai-yu |
| Institution: | WANG Kai-yu~1,ZHAO De-ming~2,GENG Yi~1,HUANG Xiao-li~1,LIU Guang-xun~3 |
| Abstract: | Hepatic cells apoptosis induced by olaquindox in common carp, Cyprinus carpio , were studied. The test was conducted for 30 days, with dose of 200mg..kg - 1 feed. Terminal deoxynucleotidy transferase mediated dUTP-Biotin nick-end labeling ( TUNEL) , electronic microscopic and flow cytometry were performed to analyze apoptosis. The result showed that hepatic cells apoptosis were indent ified under light microscope with TUNEL staining; chromatin condensation, chromosal clumping and margination, and apoptotic bodies were observed under electronic microscope; and sub-G1 PEAK ( AP peak) in hepatic cells could be detected by f low cytometric method. The apoptotic rate measured by flow cytomtry increased with dye-poison time, on the 7th, 14th, 20th, 25th and 30th day, the apoptotic rate was 2. 05%, 6. 25%, 9. 49%, 13. 31% and 17. 44% respectively. |
| Keywords: | Cyprinus carpio apoptosis olaquindox hepatic cell |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《水产学报》浏览原始摘要信息 | |
| 点击此处可从《水产学报》下载免费的PDF全文 | |