| 枣幼胚体细胞胚发生发育的组织学观察 |
| |
| 引用本文： | 任海燕,弓桂花,王永康,赵爱玲,薛晓芳.枣幼胚体细胞胚发生发育的组织学观察[J].农学学报,2019,9(3):32-35. |
| |
| 作者姓名： | 任海燕 弓桂花 王永康 赵爱玲 薛晓芳 |
| |
| 作者单位： | 山西省农业科学院果树研究所 |
| |
| 基金项目： | 国家科技资源共享服务平台子平台项目“国家枣葡萄种质资源平台”(NICGR2017-051)；“十二五”农村领域国家科技计划项目“鲜食枣和 干制枣高效生产关键技术研究与示范”(2013BAD14B03)；山西省科技重点研发项目“红枣商品化关键技术开发”(2015-TN-4)；山西省自然基金项目 “枣胚败育机理研究”(2013011034-1)。 |
| |
| 摘 要： | 为深入研究枣体细胞胚胎发生的形态建成过程,以小梨枣幼胚为材料,采用苏木精-伊红法染色和石蜡切片技术对体细胞胚发生、发育进行了组织学观察。结果表明：胚型愈伤组织细胞体积小,细胞核大,细胞质浓,细胞排列紧密；而非胚性愈伤组织细胞体积大,细胞核小,细胞质稀薄。体细胞胚产生于幼胚愈伤组织的表层细胞或内部细胞,且发生不同步,其发育过程与合子胚发育过程相似,即经历球形期、心形期和鱼雷期,最终形成子叶形胚。
|
| 关 键 词： | 乡村振兴 乡村振兴 新型农业经营主体培育 上海市浦东新区 |
| 收稿时间： | 2018-05-04 |
Somatic Embryogenesis and Development in Ziziphus jujuba Mill.: Histological Observation |
| |
| Abstract: | The paper aims to study the morphogenesis process of somatic embryogenesis. We used young embryos of Ziziphus jujuba‘Xiaolizao’as explants, and carried out the histological observation of somatic embryo formation and development by using the method of hematoxylin-eosin staining and paraffin section. The results indicated that: the embryogenic callus cells had the characteristics of small size, large nucleus, thick cytoplasm and the cells were closely arranged; however, the non- embryogenic callus cells were characterized with large size, small nucleus and thin cytoplasm. The somatic embryos were originated from surface calluscells and inner calluscells of immature embryos and their development were not synchronized which was similar to the embryonic development process of zygotes, the somatic embryos also experienced the
spherical stage, the heart-shaped stage, the torpedo stage and ultimately formed cotyledon embryos. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《农学学报》浏览原始摘要信息 |
| 点击此处可从《农学学报》下载免费的PDF全文 |
|
