| 山药腐烂微生物的分离、鉴定及抑制研究 |
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| 引用本文： | 刘少军,孙亚玲,姚甜甜,舒锐,岳林旭,臧传江,许念芳,李晓龙,焦健,彭勇.山药腐烂微生物的分离、鉴定及抑制研究[J].农学学报,2019,9(7):24-30. |
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| 作者姓名： | 刘少军 孙亚玲 姚甜甜 舒锐 岳林旭 臧传江 许念芳 李晓龙 焦健 彭勇 |
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| 作者单位： | 1.山东省轻工农副原料研究所;2.山东农业大学食品科学与工程学院 |
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| 基金项目： | 潍坊市科技发展计划项目“山药周年储藏保鲜技术研究”(2017ZJ1039)。 |
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| 摘 要： | 山药作为药食兼用性植物,含有丰富的营养成分和独特的功能。本研究以4个山药品种为试材,分离出采后山药腐烂病原微生物,通过形态学、18S rRNA序列测序和构建系统进化树的方法对分离菌株进行了生物学鉴定,结果表明,引起山药腐烂的微生物与青霉菌家族Penicillium sclerotigenum亲缘关系最近,确定该菌株为Penicillium sclerotigenum。利用不同pH(3、5、7、9、11)和不同生防剂(EM原液、多粘芽孢杆菌、哈茨木霉菌、枯草芽孢杆菌、细黄链霉菌)对山药青霉菌生长特性进行研究,结果表明,青霉菌生长最适pH值在5-7之间,培养42 h的最适pH是5；枯草芽孢杆菌和细黄链霉菌对山药腐烂微生物青霉菌有较好的抑菌效果。
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| 关 键 词： | 气候变化 气候变化 现代农业 科技传播 体系 |
| 收稿时间： | 2018-10-08 |
Isolation,Identification and Inhibition of Microorganisms Causing Yam Rotten |
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| Abstract: | The paper aims to study the rot of yam during storage. The pathogenic microorganisms causing rot of four yam cultivars after harvest were isolated in this research. The isolated strains were identified by morphological, 18S rRNA sequencing and phylogenetic tree. The results showed that the microorganisms causing rot of yam had high homogeneity with Penicillium sclerotigenum strain, which were determined to be P. sclerotigenum. The growth characteristics of P. sclerotigenum were studied by different pH (3, 5, 7, 9, 11) and different biocontrol agents (EM, Bacillus polysaccharide, Trichoderma harzianum, Bacillus subtilis and Streptomyces microflavus). The results showed that the optimum pH value of P. sclerotigenum was 5-7, and the optimum pH value after cultured 42 h was 5. The Bacillus polysaccharide and S. microflavus had better
inhibitory efficacy on P. sclerotigenum. |
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