| 体细胞克隆山羊"元元"肺的形态学观察 |
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| 引用本文： | 郑月茂,安志兴,张雅蓉,张涌.体细胞克隆山羊"元元"肺的形态学观察[J].畜牧兽医学报,2005,36(10):1091-1094. |
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| 作者姓名： | 郑月茂 安志兴 张雅蓉 张涌 |
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| 作者单位： | 西北农林科技大学,生物工程研究所,杨凌,712100 |
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| 基金项目： | 国家科技部“863”高技术研究发展规划项目（2001AA2l3081） |
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| 摘 要： | 动物体细胞克隆是指由一个体细胞产生一个和亲代遗传基因一致、形态非常相像的动物。体细胞克隆已相继在牛、小鼠、山羊、猪、野牛、马、猫、大鼠等动物上取得成功。2000年6月中旬,西北农林科技大学生物工程研究所利用成年山羊耳部皮肤成纤维细胞作为核供体,获得两只体细胞克隆山羊——“元元”和“阳阳”。“元元”因呼吸衰竭仅存活36．05h。为了了解“元元”肺的发育情况,作者对“元元”的肺进行了肉眼、光镜和透射电镜观察。
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| 关 键 词： | 肺 形态 元元 山羊 |
| 文章编号： | 0366-6964（2005）10-1091-04 |
| 收稿时间： | 2005-02-21 |
| 修稿时间： | 2005-02-21 |
Morphological Study on the Lung of Somatic Cell Cloning Goat "Yuan-yuan" |
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| ZHENG Yue-mao,AN Zhi-xing,ZHANG Ya-rong,ZHANG Yong.Morphological Study on the Lung of Somatic Cell Cloning Goat "Yuan-yuan"[J].Acta Veterinaria et Zootechnica Sinica,2005,36(10):1091-1094. |
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| Authors: | ZHENG Yue-mao AN Zhi-xing ZHANG Ya-rong ZHANG Yong |
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| Institution: | Institute of Bio-Engineering, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China |
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| Abstract: | Lung of the somatic cell cloning goat "yuan-yuan" was observed with eyes,LM and TEM,the results are as follows: The left lung have two lobes and the right lung have four lobes.The fissures are incomplete which are connected with lobes by serosa.Many epithelial cells are full of osmotin.The respiratory bronchioles were lined with cuboid cells.The alveoli epithelium differentiated into flattened(type I cell) and cuboid(type II cell)epithelial cells.The capillary endothelium were pressed close to the alveolar epithelium.The bloodair barrier was composed of type I cell,basement membrane and endothelium.The osmiophilic lamellar bodies and ribosomes are seen abundantly in type II alveolar cells,rough endoplasmic reticulum pools expand,Mitochondria denatured,microvilli of the epithelial cells appear on the luminal surface. |
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| Keywords: | lung morphology "yuan-yuan" goat |
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