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A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method
Authors:YAN Miao-miao  WEI Guang-cheng  PAN Xiao-hong  MA Huai-lei  LI Wei-zhen
Abstract:Objective] The study aimed to introduea a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. Method] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the spe-cifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. Result] The clear and orderly bands were observed in gel detection, and the val-ues of OD260/OD280 for DNAs extracted via modified CTAB method were between 1.77 - I. 83. The DNAs performed well in PCR amplification. Conclu-sion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.
Keywords:Animal  Plant  Extraction of genomic DNA  Modified CTAB method
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