| 利用PCR技术同时鉴定番茄抗根结线虫和抗斑萎病毒基因 |
| |
| 引用本文： | 李君明,宋燕,徐和金,周永健,Carole Caranta,冯兰香.利用PCR技术同时鉴定番茄抗根结线虫和抗斑萎病毒基因[J].园艺学报,2003,30(6):678-682. |
| |
| 作者姓名： | 李君明 宋燕 徐和金 周永健 Carole Caranta 冯兰香 |
| |
| 作者单位： | ( 中国农业科学院蔬菜花卉研究所，北京100081； Institute National Breeding of Fruits and Vegetables，Monfavet Avignon 84143，France) |
| |
| 基金项目： | 国家自然科学基金项目(30100124) |
| |
| 摘 要： | 利用同一PCR反应体系，对分别与番茄抗根结线虫的 基因和抗斑萎病毒(1w V)的Sw一5基因紧密连锁的SCAR标记进行了同时扩增筛选，扩增的特异性片段与单引物扩增片段完全吻合，其中与基因紧密连锁的SCAR1标记为共显性标记，抗感试材均产生750 bp的特异片段，纯合和杂合抗病基因型试材存在 I酶切位点，酶切后分别产生了570 bp、160 bp和750 bp、570 bp、160 bp的不同特异性片段，而感病基因型试材无 I酶切位点；与Sw一5基因紧密连锁的SCAR2标记为显性标记，只有抗病试材扩增出400 bD的特异性片段。经反复验证，结果稳定准确可靠，可用于在同一PCR反应体系中对两个抗病基因进行同时筛选鉴定。
|
| 关 键 词： | 番茄 分子标记 PCR Mi基因 一5基因 |
| 文章编号： | 0513-353X(2003)06-0678-05 |
| 收稿时间： | 2003-4-19 |
| 修稿时间： | 2003年4月19日 |
Simultaneous Identification of Multi-genes with Resistance Respectively to Root-knot Nematode and TSWV by PCR Markers in Tomato |
| |
| Carole Caranta.Simultaneous Identification of Multi-genes with Resistance Respectively to Root-knot Nematode and TSWV by PCR Markers in Tomato[J].Acta Horticulturae Sinica,2003,30(6):678-682. |
| |
| Authors: | Carole Caranta |
| |
| Institution: | ( Institute of and Flowers，Chinese Academy of Agricultural Sciences，Bejing 100081， China； Institute National Breeding of Fruits and Vegetables，Montfavet Avignon 84143，France) |
| |
| Abstract: | Single PCR reaction with two SCAR markers respectively tightly linked with Mi and Sw-5 genes in tomato, which resistant to root-knot nematode and tomato spotted wilt virus, has been used to screen the multiplex bands. The PCR products with multiplex primers were completely correspond to the amplified bands produced by single SCAR primer. Among them, codominant SCAR1 marker tightly linked with Mi gene produce 750 bp fragment in both resistant and susceptible tomato lines. The amplified bands from susceptible and resistant lines were distinguishable after cleavage with the restriction enzyme TaqI. Genotype with homozygous and heterozygous Mi gene could produce respectively 570 bp, 160 bp and 750, 570 bp, 160 bp bands. Susceptible genotypes still present 750 bp fragment. The dominant SCAR2 marker tighdy linked with Sw-5 gene would produce only 400 bp PCR product in resistant genotype. The replicated stable results proved that two resistant genes could be identified simultaneously by using corresponding SCAR primer under adaptable condition. This system compared with single primer PCR would be time saving, less labor and low cost. It could be very useful for marker-assisted selection during early stage in tomato and efficiently speed up breeding procedure. |
| |
| Keywords: | Tomato PCR Mi gene Sw-5 gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
