| 大叶栎总DNA两种提取方法的比较 |
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| 引用本文： | 梁文汇,梁机,HUANG Shou-xian,黄寿先,谢素平,周传明.大叶栎总DNA两种提取方法的比较[J].广西林业科学,2007,36(1):50-52. |
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| 作者姓名： | 梁文汇 梁机 HUANG Shou-xian 黄寿先 谢素平 周传明 |
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| 作者单位： | 广西大学林学院,南宁,530005 |
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| 摘 要： | 对大叶栎的总DNA两种提取方法进行对比试验,从外观、浓度、A260/A280、A260/A230、电泳检查和ISSR扩增对所提取到的DNA进行对比,结果显示用改良CTAB法提取的DNA在各方面都达到ISSR扩增的要求,是大叶栎总DNA提取的较好方法。
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| 关 键 词： | 大叶栎 DNA提取 ISSR 改良CTAB法 |
| 文章编号： | 1006-1126(2007)01-0050-03 |
Comparison of Two Total DNA Extraction Methods for Castanopsis fissa |
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| HUANG Shou-xian.Comparison of Two Total DNA Extraction Methods for Castanopsis fissa[J].Guangxi Forestry Science,2007,36(1):50-52. |
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| Authors: | HUANG Shou-xian |
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| Institution: | Forestry College of Guangxi University , Nanning 530005 , Guangxi |
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| Abstract: | Two total DNA extraction methods of Casta nopsis fissa were compared in the paper.By studying the appearance,concentration,A260/A280,A260/A230 and electrophoresis,and comparing the total DNA extracted from ISSR analysis,the result showed that the improved CTAB method was suitable for ISSR analysis,and it was a better DNA extraction method of Casta nopsis fissa. |
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| Keywords: | Casta nopsis fissa dNA extraction ISSR improved CTAB method |
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