| 棉枯萎镰刀菌血清学检测方法和技术的研究 |
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| 引用本文： | 李启新,杜琦,陈长法,李祥.棉枯萎镰刀菌血清学检测方法和技术的研究[J].中国农业大学学报,1998(Z1). |
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| 作者姓名： | 李启新 杜琦 陈长法 李祥 |
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| 作者单位： | 青岛动植物检疫局;华中农业大学植保系 青岛;266002; |
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| 摘 要： | 以棉枯萎镰刀菌公安菌株为材料,用物理法和化学法提取抗原并制备抗血清,并选有代表性的抗血清对其特异性和灵敏度分别采用4种检测方法进行了检测比较研究。结果表明:(1)采用 Richard’s培养基振荡培养菌株,化学法提取菌丝孢子抗原,按改进的 J.E.Devay 设计的免疫方案免疫,能产生效价高达1:64的抗血清。(2)琼扩、比较对流免疫电泳、间接 ELISA,SPA-ELISA 等4种方法检测其特异性具有鉴别到尖孢镰刀菌种的水平的特异性,但不能鉴别专化型和生理小种。并用上述方法检测抗血清灵敏度。
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| 关 键 词： | 棉枯萎病 血清学 琼脂双扩散 对流免疫电泳 间接 ELISA SPA-ELISA |
Studies on the Methods and Techniques for Serodetection of Fusarium oxysporum Schl.f.sp.vasinfectum(Atk.)Snyder et Hansen |
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| Li Qixin Du Qi Chen Changfa ,Qingdao,Li Xiang.Studies on the Methods and Techniques for Serodetection of Fusarium oxysporum Schl.f.sp.vasinfectum(Atk.)Snyder et Hansen[J].Journal of China Agricultural University,1998(Z1). |
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| Authors: | Li Qixin Du Qi Chen Changfa Qingdao Li Xiang |
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| Institution: | Li Qixin Du Qi Chen Changfa (Qingdao Animal & Plant Quarantine Service(QAPQ),Qingdao,266002)Li Xiang (Middle China Agricultural University) |
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| Abstract: | The pathogen Fusarium oxysporum f.sp.vasinfectumGong-anstrain of cotton fusarium wilt was tested.The antigen protein was extracted by physical and chemical methods and antisera were produced with Japanese white rabbits.Two representative anti- sera As2 and As10 were used to study their specificity and sensitivity with four methods. The results indicated that:(1)The strains were cultured vibrantly in Richard's medium. Extracts from mycelia and conidia were treated with chemical method and immuned rabbits as ... |
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| Keywords: | cotton fusarium wilt serology agar dimension diffusion(ADD) immuno-osmophoresis indirect ELISA SPA-ELISA |
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