| 甘肃新选育党参品系与主栽品种的RAPD分析 |
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| 引用本文： | 杨宁,陈锡莲,高新彦,史万斌,刘效瑞.甘肃新选育党参品系与主栽品种的RAPD分析[J].草业科学,2012,29(12):1883-1886. |
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| 作者姓名： | 杨宁 陈锡莲 高新彦 史万斌 刘效瑞 |
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| 作者单位： | 西北师范大学生命科学学院,甘肃兰州,730070;兰州市城关区徐家山林场,甘肃兰州,730000;定西市旱作农业科研推广中心,甘肃定西,743000 |
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| 基金项目： | 国家自然科学基金，甘肃省自然科学基金项目，甘肃省教育厅研究生导师项目，甘肃省农牧厅项目，西北师范大学学生科研资助金项目 |
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| 摘 要： | 采用RAPD分子标记技术，分析了新选育党参（Codonopsis pilosula）8个品系和2个主栽品种的遗传多样性和亲缘关系。结果显示，14条引物共扩增出159个位点，其中多态位点26个，多态性位点比率为46.43%，材料间遗传相似系数范围在0.771 9～0.964 9，说明甘肃省主栽党参品种资源具有较丰富的遗传多样性。根据聚类分析结果，党参品种DS6与所有其他供试材料的亲缘关系最远，可单独聚为一类；DS3与DS8的亲缘关系最近，其相似系数为0.964 9。
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| 关 键 词： | 党参 RAPD 遗传多样性 多态住点 |
RAPD analysis of different major cultivated species of Codonopsis pilosula in Gansu |
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| YANG Ning,CHEN Xi lian,GAO Xin yan,SHI Wan bin,LIU Xiao rui.RAPD analysis of different major cultivated species of Codonopsis pilosula in Gansu[J].Pratacultural Science,2012,29(12):1883-1886. |
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| Authors: | YANG Ning CHEN Xi lian GAO Xin yan SHI Wan bin LIU Xiao rui |
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| Abstract: | Genetic differences and relationship of 10 (8 lines and 2 cultivars) Codonopsis pilosula species in Gansu were examined by RAPD in this study. The results showed that a total of 159 DNA bands were amplified by 14 oligonucleotide primers, of which 26 bands were polymorphic. The rate of polymorphism was 45.61%. According to the cluster analysis, the 10 C. pilosula species had genetic similarity coefficients ranged from 0.771 9 to 0.964 9. The genetic relationship of DS6 species was far from others. However, the genetic relationship between DS3 and DS8 was close with a similarity coefficient of 0.964 9. |
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| Keywords: | Codonopsis pilosula RAPD genetic diversity polymorphism |
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