| 枣子叶再生植株研究 |
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| 引用本文： | 郭敬丽,王玖瑞,刘孟军,代丽.枣子叶再生植株研究[J].河北农业大学学报,2009,32(4). |
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| 作者姓名： | 郭敬丽 王玖瑞 刘孟军 代丽 |
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| 作者单位： | 1. 河北农业大学,林学院,河北,保定,071001
2. 河北农业大学,中国枣研究中心,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金，河北省科技支撑计划，河北农业大学科技将帅项目和河北农业大学9816项目 |
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| 摘 要： | 以枣树‘雄性不育3号’未成熟胚的子叶为材料,建立了枣子叶再生植株体系。子叶愈伤诱导培养基为MS+蔗糖30 g/L+琼脂3.3 g/L,愈伤诱导率达到40%。从愈伤组织诱导不定芽的培养基为MS+蔗糖30 g/L+琼脂3.3 g/L+TDZ 1.5 mg/L,不定芽发生率为88.9%,不定芽数量与愈伤数量之比为3.5。生根培养基为MS+蔗糖30 g/L+琼脂3.0 g/L+IBA 1.0 mg/L,生根率达到33.3%。此外,培养温度显著影响玻璃化苗的生长。在24℃条件下培养30 d,仅有9.7%的玻璃化苗向正常植株转化,而在20℃培养条件下的玻璃化率仅为6.7%。附加30~80 g/L蔗糖的不同培养基上,玻璃化苗向正常苗转化情况无显著差异。
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| 关 键 词： | 枣 子叶 植株再生 玻璃化 |
Studies on plant regeneration from cotyledon of Chinese jujube |
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| GUO Jing-li,WANG Jiu-rui,LIU Meng-jun,DAI Li.Studies on plant regeneration from cotyledon of Chinese jujube[J].Journal of Agricultural University of Hebei,2009,32(4). |
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| Authors: | GUO Jing-li WANG Jiu-rui LIU Meng-jun DAI Li |
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| Institution: | GUO Jing-li1,WANG Jiu-rui1,LIU Meng-jun2,DAI Li2 (1.College of Forestry,Agricultural University of Hebei,Baoding 071001,China,2.Research Center of Chinese Jujube,China) |
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| Abstract: | Plant regeneration system was established using cotyledons in immature fruits of Chinese jujube male sterile No.3('JMS3').Callus could be induced from cotyledon of 'JMS3' in medium MS+sugar 30 g/L+agar 3.3 g/L without hormones and the callus rate was 40%.Adventitious buds occurred in 88.9% of callus cultured in medium MS+sugar 30 g/L+agar 3.3 g/L+TDZ 1.5 mg/L and the amount of adventitious buds is 3.5 times callus.Rooting medium was MS +sugar 30 g/L+agar 3.3 g/L+IBA 1.0 mg/L and the rooting rate was 33.3%.I... |
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| Keywords: | Chinese jujube cotyledon plant regeneration vitrification |
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