| TGEV与PEDV核衣壳蛋白双价基因原核质粒的构建与表达 |
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| 引用本文： | 王劭,陈少莺,朱小丽,程晓霞,林锋强,陈仕龙,李兆龙,朱晓琳.TGEV与PEDV核衣壳蛋白双价基因原核质粒的构建与表达[J].福建农业学报,2011,26(1):1-5. |
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| 作者姓名： | 王劭 陈少莺 朱小丽 程晓霞 林锋强 陈仕龙 李兆龙 朱晓琳 |
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| 作者单位： | 王劭,陈少莺,程晓霞,林锋强,陈仕龙,WANG Shao,CHEN Shao-ying,CHENG Xiao-xia,LIN Feng-qian,CHENG Shi-long(福建省农业科学院畜牧兽医研究所,福建,福州,350013;福建省畜禽疫病防治工程技术研究中心,福建,福州,350013);朱小丽,李兆龙,ZHU Xiao-li,LI Zhao-long(福建省农业科学院畜牧兽医研究所,福建,福州,350013);朱晓琳,ZHU Xiao-Lin(福建农林大学动物科学学院,福建,福州,350002) |
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| 基金项目： | 福建省财政专项，福建省属公益类科研院所基本科研专项 |
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| 摘 要： | 运用DNA重组技术将猪传染性胃肠炎病毒核衣壳基因、猪流行性腹泻病毒核衣壳基因编码最大局部亲水性抗原决定簇基因片段进行串联,克隆到原核表达载体pET-32a(+)中,构建预防仔猪冠状病毒性腹泻双价基因原核表达质粒.将该质粒转化至大肠杆菌BL21(DE3)中,经IPTG诱导后,SDS-PAGE电泳结果显示,双价重组基因表达...
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| 关 键 词： | 猪传染性胃肠炎病毒 猪流行性腹泻病毒 核衣壳蛋白 原核表达 共表达 |
Prokaryotic expression plasmid with nucleocapsid genes of TGEV/PEDV and co-expression of two genes |
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| WANG Shao,CHEN Shao-ying,ZHU Xiao-li,CHENG Xiao-xia,LIN Feng-qian,CHENG Shi-long,LI Zhao-long,ZHU Xiao-Lin.Prokaryotic expression plasmid with nucleocapsid genes of TGEV/PEDV and co-expression of two genes[J].Fujian Journal of Agricultural Sciences,2011,26(1):1-5. |
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| Authors: | WANG Shao CHEN Shao-ying ZHU Xiao-li CHENG Xiao-xia LIN Feng-qian CHENG Shi-long LI Zhao-long ZHU Xiao-Lin |
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| Institution: | WANG Shao1,2,CHEN Shao-ying1,ZHU Xiao-li1,CHENG Xiao-xia1,LIN Feng-qian1,CHENG Shi-long1,LI Zhao-long1,ZHU Xiao-Lin(1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences,Fuzhou,Fujian 350013,China,2.Fujian Animal Diseases Control Technology Development Center,3.College of Animal Science,Fujian Agriculture and Forestry University,Fujian 350002,China) |
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| Abstract: | By using DNA recombination technology,nucleocapsid gene from swine transmissible gastroenteritis virus was lined with the gene fragment of the maximum local hydrophilic antigenic determinant of nucleocapsid gene from porcine epidemic diarrhea virus.The recombinant gene was cloned into the prokaryotic expression vector pET-32a(+).Then,the prokaryotic expression plasmid with the bivalent gene to prevent piglet's coronaviral diarrhea was constructed.After transforming the plasmid into E.coil BL21(DE3),the bact... |
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| Keywords: | swine transmissible gastroenteritis virus porcine epidemic diarrhea virus nucleocapsid protein prokaryotic expression co-expression |
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