Standardization of Ultraviolet Irradiation of Channel Catfish Sperm |
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| Authors: | J Michael Christensen Terrence R Tiersch |
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| Institution: | School of Forestry, Wildlife. and Fisheries, Louisiana Agricultural Experiment Station. Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 USA |
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| Abstract: | Gynogenesis is a naturally occurring phenomenon in lower vertebrates in which offspring receive two sets of chromosomes from the female (Dawley 1989). This occurs when development is activated by sperm, but genetic material from the male is not incorporated into the embryo. Normally, haploid offspring result that do not survive to hatching, although in certain instances suppression of meiosis II or first mitosis can result in diploid gynogenetic offspring (Purdom 1993). Artificially induced gynogenesis has been used in the breeding and genetic study of several fishes, including salmonids (Chourrout 1982; Allendorf et al. 1986), tilapia (Don and Avtalion 1988), and channel catfish (Liu et al. 1992). Gynogenesis has been applied to production of monosex populations, isogenic populations, and inbred lines of fish (Ihssen et al. 1990; Tave 1993). Gynogenesis can be induced artificially by irradiating sperm with ultraviolet (UV) radiation to inactivate genetic material. Eggs are fertilized with the irradiated sperm and shocked by temperature or pressure change to restore diploidy. |
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