| 半定量RT-PCR法检测低磷胁迫下大豆根系质膜H~+-ATPase基因的表达 |
| |
| 引用本文： | 张洁,张晓红,张振海,韩胜芳,王冬梅.半定量RT-PCR法检测低磷胁迫下大豆根系质膜H~+-ATPase基因的表达[J].河北农业大学学报,2009,32(4). |
| |
| 作者姓名： | 张洁 张晓红 张振海 韩胜芳 王冬梅 |
| |
| 作者单位： | 1. 河北农业大学,生命科学学院,河北,保定,071001
2. 保定市农业局,河北,保定,071051 |
| |
| 基金项目： | 河北省科技攻关计划项目，河北农业大学科研发展基金，河北省教育厅资助项目 |
| |
| 摘 要： | 试验以大豆为材料,采用水培方法,以β-微管蛋白基因为内参基因,用半定量逆转录聚合酶链式反应(se-mi RT-PCR法)检测在低磷胁迫下大豆根系质膜H+-ATPase基因表达量的变化,以期建立适于检测该基因表达的semi RT-PCR试验体系。结果表明:在低磷胁迫2 h时,与对照相比基因表达量有所增加,在4 h时相对表达量达到最大,6 h略有下降。这表明大豆根系质膜H+-ATPase基因表达量的增加可能与适应低磷胁迫的逆境有关,半定量RT-PCR法可以用来检测特定基因在不同条件下的表达量。
|
| 关 键 词： | 大豆 半定量RT-PCR 低磷胁迫 质膜H+-ATPase |
Expression of plasma membrane H~+-ATPase in soybean root by semi RT-PCR systems under low phosphorus stress |
| |
| ZHANG Jie,ZHANG Xiao-hong,ZHANG Zhen-hai,HAN Sheng-fang,WANG Dong-mei.Expression of plasma membrane H~+-ATPase in soybean root by semi RT-PCR systems under low phosphorus stress[J].Journal of Agricultural University of Hebei,2009,32(4). |
| |
| Authors: | ZHANG Jie ZHANG Xiao-hong ZHANG Zhen-hai HAN Sheng-fang WANG Dong-mei |
| |
| Institution: | ZHANG Jie1,ZHANG Xiao-hong2,ZHANG Zhen-hai1,HAN Sheng-fang1,WANG Dong-mei1 (1.Lifes Science College Agricultural University of Hebei,Baoding 071001,China,2.Baoding Agricultural Bureau,Baoding 071051,China) |
| |
| Abstract: | In this experiment, a semi RT-PCR system was established to detect the gene expression of PM H+-ATPase in soybean root under phosphorus deficiency quickly and conveniently, in which β-tubulin gene was used as reference gene. The results showed that the gene of PM H+-ATPase was up-regulated under low P environment for 2 h and increased gradually until 4 h, but the gene expression decreased a little as the time delayed to 6 h. It was shown that gene expression changes of PM H+-ATPase may be related to the adaptation of the phosphorus deficiency stress, furthermore, the semi-quantitative RT-PCR system could be used to detect gene expression under different conditions successfully. |
| |
| Keywords: | soybean[Glycine max(L )Merrill] semi-quantitative PCR phosphorus deficiency stress plasma membrane H+-ATPase |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
