| 梨花柱半离体培养法及品种自交不亲和基因型鉴定 |
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| 引用本文： | 张绍铃,周建涛,徐义流,陈迪新,徐国华,吴桂法.梨花柱半离体培养法及品种自交不亲和基因型鉴定[J].园艺学报,2003,30(6):703-706. |
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| 作者姓名： | 张绍铃 周建涛 徐义流 陈迪新 徐国华 吴桂法 |
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| 作者单位： | ( 南京农业大学园艺学院，南京210095； 高邮市果树实验场，高邮2256oo) |
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| 基金项目： | 国家自然科学基金项目(30170651);高校博士学科点专项科研基金项目(20010307012);江苏省自然科学基金项目(BK2001062);江苏省高技术研究计划项目(BG2002309) |
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| 摘 要： | ：切取梨授粉花柱进行半离体培养的结果表明，杂交不亲和性组合从花柱基部长出花粉管的花柱比率为0～32．4％ ，且大多在16％ 以下，而杂交亲和性组合为51．6％ ～86．7％ ，与田间杂交授粉的坐果率相近，且在授粉花粉的萌发率达20％以上时，温室水培枝条促成的花与露地开花的花之间没有显著差异。应用此方法鉴定出了梨品种‘筑水’、‘喜水’及‘爱甘水’的s基因型分别为s s 、s s 、及s s 。
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| 关 键 词： | 梨 杂交不亲和 杂交亲和 半离体培养法 基因型 |
| 文章编号： | 0513-353X(2003)06-0703-04 |
| 收稿时间： | 2003-4-18 |
| 修稿时间： | 2003年4月18日 |
Semi Vitro Culture of Pear Style and Identification of the Genotypes of Pear Self-incompatibility |
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| Zhang Shading,Zhou Jiantao,Xu Yiliu,Chen Dixin,Xu Guohua,and Wu Guifa.Semi Vitro Culture of Pear Style and Identification of the Genotypes of Pear Self-incompatibility[J].Acta Horticulturae Sinica,2003,30(6):703-706. |
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| Authors: | Zhang Shading Zhou Jiantao Xu Yiliu Chen Dixin Xu Guohua and Wu Guifa |
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| Institution: | ( College ofHorticulture，Nanjing Agricultural Utdversity，Nanjing 210095，China； Go~ou Orchard，G~2oyoll 225600， Ch／na ) |
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| Abstract: | The results of semi vitro culturing pollinated styles excised from plants indicated that the amount of pollen tubes from style bottoms and the percentage of styles with pollen tubes through their bottoms in all tested styles differed with the cross combinations including the incompatible combination and compatible combination. For the former ones, the percentage ranged from 0 to 32.4%, most of which were under 16%, while for the latter ones, the percentage ranged from 51.6% to 86.7% that was close to the rate of field fruit setting after cross-pollination. Furthermore, when the pollen germination rate was above 20% , there was no significant difference between the flowers from the room water cultured flowering woods and field orchards as for the percentage of styles with pollen tubes through their bottoms in all styles. So we can extend the time span of collecting materials for our experiments. For the first time, the method of semi vitro culturing pollinated pear styles including cross compatible and cross incompatible was reported. We have also identified the genotypes of pear cultivars Chikusui, Kisui and Aikansui, namely S3S4,S4S5 and S4S5, respectively. |
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| Keywords: | Pear Cross-incompatibility Cross-compatibility Semi vitro culture Genotype |
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