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In vitro morphogenetic capacity of Nicotiana tabacum L. var. BEL W3 upon cyclic culture and regeneration
Authors:Silvia Schiff  Andrea Bennici
Institution:(1) Plant Biology Department, University of Florence, Piazzale delle Cascine 28, 50144 Florence, Italy
Abstract:Summary Variation in the capacity to regenerate shoots and morphogenetic potential were investigated by cyclic culture and regeneration of Nicotiana tabacum var. BEL W3. Explants were excised from different shoots formed by the same callus line. Differences in the organogenetic in vitro response among regenerated plantlets was observed in two subsequent regenerate lsquogenerationsrsquo. A progressive decrease of the regenerative capacity was observed across callus line lsquogenerationsrsquo.
Keywords:Nicotiana tabacum var  BEL W3  tobacco  callus  morphogenetic capacity  somaclone  epigenetic
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