| 葛根素对草鱼非特异性免疫机能和肝胰脏组织结构的影响 |
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| 引用本文： | 饶毅.葛根素对草鱼非特异性免疫机能和肝胰脏组织结构的影响[J].中国农学通报,2017,33(26):136-140. |
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| 作者姓名： | 饶毅 |
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| 作者单位： | 江西省水产科学研究所 |
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| 基金项目： | 江西省现代农业科研协同创新专项“草鱼池塘健康生态高效养殖技术研究”(JXXTCX2015004-006)；江西省现代农业产业技术体系专项 (JXARS-03)；江西省农牧渔科研指导性计划项目“不同饲料投喂量下生物絮团技术对草鱼生长的影响”(2016123-17)。 |
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| 摘 要： | 为研究葛根提取物葛根素对草鱼非特异性免疫指标和肝胰脏组织结构的影响,在基础饲料(对照组)中分别添加10、50、250、1250 mg/kg的葛根素,饲养平均体重(4.52±0.22)g的草鱼。经28天的饲养,结果表明,与对照组比较,10 mg/kg葛根素添加组碱性磷酸酶活性显著高于对照组(P0.05),10、50 mg/kg葛根素添加组超氧化物歧化酶显著高于对照组(P0.05),溶菌酶各组间无显著差异。肝胰脏指数各试验组与对照组无显著差异。对照组和各试验组之间肝胰脏组织结构差异也不显著。饲喂结束后,利用嗜水气单胞菌人工感染草鱼,各试验组的相对免疫保护率都提高,但10 mg/kg葛根素添加组对草鱼的保护率显著高于对照组(P0.05),免疫保护率达73.33%。试验表明,在饲料中添加一定量的葛根素(10 mg/kg)能提高草鱼的非特异性免疫机能,且不会造成肝胰脏组织结构的损伤。
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| 关 键 词： | 葛根素 草鱼 非特异性免疫 肝胰脏指数 肝胰脏组织结构 |
| 收稿时间： | 2017/1/16 0:00:00 |
| 修稿时间： | 2017/5/3 0:00:00 |
Puerarin Affecting Non-specific Immune Function and Hepatopancreas Structure of Grass Carp (Ctenopharyngodon idellus) |
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| Abstract: | The study aims to research the effects of adding puerarin in diets on non-specific immune function and hepatopancreas structure of grass carp (Ctenopharyngodon idellus). C. idellus with body weight of (4.52±0.22) g were fed with basal diet without (control group) or with 10, 50, 250, and 1250 mg/kg puerarin extract for 28 days. The results showed that the serum AKP activity significantly increased in the group with 10 mg/kg puerarin addition compared with that in control group (P<0.05), and SOD activity significantly increased in the groups with 10 and 50 mg/kg puerarin addition (P<0.05). However, LZM activity was not affected in all the tested groups. Hepatopancreas index and structure was not significantly different between tested groups and control group. After the feeding experiment, C. idellus were infected with Aeromonas hydrophila. Survival rates in the test groups all increased, and the increase was significant for the group with 10 mg/kg puerarin addition (immune protective rate of 73.33%, P<0.05). The results indicate that non-specific immune function can be improved by adding 10 mg/kg puerarin in diet, which cannot lead to hepatopancreas injury. |
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| Keywords: | Puerarin grass carp non-specific immune function hepatopancreas index hepatopancreas Structure |
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