| 我国北方潮土玉米不同生长时期古菌群落结构和丰度的变化 |
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| 引用本文： | 王晶,王蕊,朱珂,修伟明,赵建宁,杨殿林,李刚,田秀平.我国北方潮土玉米不同生长时期古菌群落结构和丰度的变化[J].玉米科学,2017,25(6):153-160. |
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| 作者姓名： | 王晶 王蕊 朱珂 修伟明 赵建宁 杨殿林 李刚 田秀平 |
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| 作者单位： | 天津农学院农学与资源环境学院, 天津 300384;农业部产地环境质量重点实验室/农业部环境保护科研监测所, 天津 300191,天津农学院农学与资源环境学院, 天津 300384;农业部产地环境质量重点实验室/农业部环境保护科研监测所, 天津 300191,天津农学院农学与资源环境学院, 天津 300384;农业部产地环境质量重点实验室/农业部环境保护科研监测所, 天津 300191,农业部产地环境质量重点实验室/农业部环境保护科研监测所, 天津 300191,农业部产地环境质量重点实验室/农业部环境保护科研监测所, 天津 300191,农业部产地环境质量重点实验室/农业部环境保护科研监测所, 天津 300191,农业部产地环境质量重点实验室/农业部环境保护科研监测所, 天津 300191,天津农学院农学与资源环境学院, 天津 300384 |
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| 基金项目： | 转基因生物新品种培育重大专项（2015ZX08013002-004，2016ZX08012005-005）、公益性行业（农业）科研专项（201503121-04）、国家自然科学基金项目（31200424） |
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| 摘 要： | 采用16S rRNA基因末端限制性片段长度多态性分析(T-RFLP)和实时荧光定量PCR技术,研究玉米不同生长时期土壤古菌群落组成和丰度的变化。结果表明,不同生长时期,根际土和非根际土古菌优势种群无显著变化,古菌群落组成无明显分离。乳熟期和完熟期古菌Shannon指数显著高于拔节期和抽雄期(P0.05),根际土抽雄期古菌Evenness指数显著低于其他生长时期(P0.05),非根际土不同生长时期Evenness指数差异不显著(P0.05)。土壤古菌16S rRNA基因丰度随生长时期的推进呈先升高后降低的趋势,乳熟期最高,拔节期最低,乳熟期显著高于拔节期和完熟期(P0.05),与抽雄期差异不显著(P0.05)。同一生长时期根际土和非根际土间无显著差异(P0.05)。
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| 关 键 词： | 玉米 16S rRNA基因 T-RFLP qPCR 群落结构 丰度 |
| 收稿时间： | 2017/3/28 0:00:00 |
Community Structure and Abundance of Soil Archaea in the Fluvo-aquic Mazie Soil in Different Growing Stages in Northern China |
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| WANG Jing,WANG Rui,ZHU Ke,XIU Wei-ming,ZHAO Jian-ning,YANG Dian-lin,LI Gang and TIAN Xiu-ping.Community Structure and Abundance of Soil Archaea in the Fluvo-aquic Mazie Soil in Different Growing Stages in Northern China[J].Journal of Maize Sciences,2017,25(6):153-160. |
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| Authors: | WANG Jing WANG Rui ZHU Ke XIU Wei-ming ZHAO Jian-ning YANG Dian-lin LI Gang and TIAN Xiu-ping |
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| Institution: | College of Agriculture and Environmental Resources, Tianjin Agriculture University, Tianjin 300384;Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China,College of Agriculture and Environmental Resources, Tianjin Agriculture University, Tianjin 300384;Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China,College of Agriculture and Environmental Resources, Tianjin Agriculture University, Tianjin 300384;Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China,Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China,Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China,Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China,Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China and College of Agriculture and Environmental Resources, Tianjin Agriculture University, Tianjin 300384 |
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| Abstract: | The molecular biological methodsterminal restriction fragment length polymorphism(T-RFLP) and quantitative real-time PCR(qPCR) technique] that based on the 16S rRNA gene were used to determine the community and abundance change of archaea in maize soil in different growth stages. The results showed that, no significant changes were observed in the dominant populations of rhizosphere and non-rhizosphere in different growth stages. There was no significant separation of the archaeal community composition. The Shannon indexes at milky stage and ripening stage were significantly higher than that of jointing stage and tassel stage(P<0.05), and the Evenness index of rhizosphere at jointing stage was significantly lower than that of other three stages(P<0.05). The abundance of archaeal 16S rRNA gene in rhizosphere and non-rhizosphere both increased the first and then decreased with the growth stages, the highest value appeared in milk stage, and the lowest appeared in jointing stage. The abundance of archaeal 16S rRNA gene in milk stage was significantly higher than that in jointing stage and ripening stage(P<0.05), but there was no significant difference with tassel stage(P>0.05). For the same growth stage, rhizosphere and non-rhizosphere showed no significant difference(P>0.05). |
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| Keywords: | Maize 16S rRNA gene T-RFLP qPCR Community structure Abundance |
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