| 文昌鸡肉质相关基因多态性分析 |
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| 引用本文： | 顾华兵,李慧芳,朱文奇,宋卫涛,韩威,徐文娟,陈宽维.文昌鸡肉质相关基因多态性分析[J].中国家禽,2011,33(18). |
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| 作者姓名： | 顾华兵 李慧芳 朱文奇 宋卫涛 韩威 徐文娟 陈宽维 |
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| 作者单位： | 江苏省家禽科学研究所,江苏扬州,225125 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(CARS-42-G03) |
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| 摘 要： | 为了探究与肉质风味相关基因A -FA BP和ADSL基因在文昌鸡群体中的遗传变异情况,本研究运用连接酶检测反应的方法(PCR-LDR)对文昌鸡A-FA BP基因C51T变异位点和ADSL基因C3484T变异位点进行基因分型,并分析了这两个位点在文昌鸡群体中的基因型、基因频率和哈-温平衡情况.结果显示,A -FABP基因C51T和ADSL基因C3484T位点均检测到CC、TT和CT 3种基因型,A-FABP基因C51T基因型频率(CC、TT和CT)和等位基因频率(C、T)分别为0.11、0.37、0.52和0.37、0.63；ADSL基因C3484T基因型频率(CC、TT和CT)和等位基因频率(C、T)分别为0.40、0.45、0.15和0.63、0.37；文昌鸡在这两个变异位点均处于哈-温平衡状态(P＞0.05).
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| 关 键 词： | 文昌鸡 脂肪细胞型脂肪酸结合蛋白基因 腺苷琥珀酸裂解酶 连接酶检测反应 |
Single Nucleotide Polymorphism Analysis of Meat Quality Candidate Genes in Wenchang Chicken |
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| GU Huabing,LI Huifang,ZHU Wenqi,SONG Weitao,HAN Wei,XU Wenjuan,CHEN Kuanwei.Single Nucleotide Polymorphism Analysis of Meat Quality Candidate Genes in Wenchang Chicken[J].China Poultry,2011,33(18). |
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| Authors: | GU Huabing LI Huifang ZHU Wenqi SONG Weitao HAN Wei XU Wenjuan CHEN Kuanwei |
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| Institution: | GU Huabing,LI Huifang,ZHU Wenqi,SONG Weitao,HAN Wei,XU Wenjuan,CHEN Kuanwei**(Jiangsu Institute of Poultry Science,Yangzhou,Jiangsu 225125) |
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| Abstract: | In order to investigate the genetic variations of two genes of A-FABP and ADSL in Wenchang chicken breed,two SNPs of A-FABP C51T and ADSL C3484T were genotyped by PCR-LDR method,the frequencies of genotypes and alleles were analyzed,and the Hardy-Weinberg equilibrium of Wenchang chicken at two SNPs sites was also detected.The results showed that there were three genotypes(CC,TT and CT) found in each mutation site;the frequencies of CC genotype,TT genotype and CT genotype at A-FABP C51T mutation site were 0.... |
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| Keywords: | Wenchang chicken A-FABP ADSL LDR |
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