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海兰鸡PRL、OVR、OIH和GnRHR基因多态性及其与产蛋性能的关联分析
引用本文:朱凯,陈强,张向喆,王起山,涂盈盈,潘玉春,杨长锁.海兰鸡PRL、OVR、OIH和GnRHR基因多态性及其与产蛋性能的关联分析[J].中国家禽,2011,33(19).
作者姓名:朱凯  陈强  张向喆  王起山  涂盈盈  潘玉春  杨长锁
作者单位:1. 上海交通大学农业与生物学院,上海200240;上海市兽医生物技术重点实验室,上海200240
2. 国家家禽工程技术研究中心,上海,200331
基金项目:国家高技术研究发展计划(863计划)项目
摘    要:本研究以海兰褐与海兰白两个蛋鸡品系作为研究素材,选择催乳素基因(PRL)、卵细胞卵黄受体基因(OVR)、卵抑制剂基因(OIH)以及促性腺激素释放激素受体基因(GnRHR)4个基因作为产蛋性状相关候选基因,采用PCR-RFLP方法对候选基因进行多样性检测,并与蛋重及产蛋数两性状进行关联分析.结果表明,PRL、OVR和OIH3个基因候选位点在本研究海兰蛋鸡群体中存在SNPs,但3个基因候选位点不同基因型与产蛋性状关联分析均未能达到显著水平(P>0.05);GnRHR基因候选位点在本研究群体中未检测到多态性的存在.

关 键 词:海兰蛋鸡  候选基因  SNPs  产蛋性状  关联分析

Association Analysis of PRL,OVR,OIH and GnRHR Gene's Polymorphisms with Laying Performances in Hy-line Chicken
ZHU Kai,CHEN Qiang,ZHANG Xiangzhe,WANG Qishan,TU Yingying,PAN Yuchun,YANG Changsuo.Association Analysis of PRL,OVR,OIH and GnRHR Gene's Polymorphisms with Laying Performances in Hy-line Chicken[J].China Poultry,2011,33(19).
Authors:ZHU Kai  CHEN Qiang  ZHANG Xiangzhe  WANG Qishan  TU Yingying  PAN Yuchun  YANG Changsuo
Institution:ZHU Kai~1,2,CHEN Qiang~1,ZHANG Xiangzhe~1,WANG Qishan~1,TU Yingying~3,PAN Yuchun~1,YANG Changsuo~3**(1.College of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,2.Shanghai Municipality Key Laboratory of Veterinary Biotechnology,3.National Poultry Engineering Research Center,Shanghai 200331)
Abstract:In this study,Hy-line brown and white laying hens were used as research materials.Prolactin gene(PRL),occytevitellogenesis receptor gene(OVR),ovoinhibitor gene(OIH)and gonadotropin-releasing hormone receptor gene(GnRHR) were employed as the candidate genes associated with laying traits.Diversity of candidate genes were detected using PCR-RFLP,then the detecting results were analyzed with egg weight and egg number.The results showed that SNPs existed at candidate site of PRL,OVR and OIH of Hy-line laying hen...
Keywords:Hy-line laying hens  candidate genes  SNPs  laying performance  association analysis  
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