Genetic similarities among Spanisch populations of Agropyron, Elymus and Thinopyrum, using PCR-based markers |
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| Authors: | Pilar García Juan-Vicente Monte Carlos Casanova Consuelo Soler |
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| Institution: | (1) I.M.I.A, El Encín, Instituto Madrileño de Investigación Agraria, 28800, Alcalá de Henares, Madrid, Spain;(2) C.I.T., I.N.I.A, Department of Plant Breeding and Biotechnology, La Canaleja, 28800, Alcalá de Henares, Madrid, Spain |
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| Abstract: | Arbitrary oligonucleotides were used as primers to amplifygenomic DNA of 48 wild Spanish populations of Agropyroncristatum, Elymus hispanicus,E. caninus,E. repens,Thinopyrum curvifolium, Th.junceum and Th.intermedium. Genetic diversity was analysedusing nineteen primers. The number of amplified products ranged from8 to 18 per primer and a total of 240 markers were scored. Differentlevels of intraspecific genetic diversity were found, the allogamousspecies E. repens andTh. intermedium being themost variable. Jaccard's similarity coefficients for internalmeasure within and between populations were used to produce a clusterdiagram. The results demonstrate differences in the degree ofsimilarity between taxonomic units. Interpopulational variability andinterspecific genomic relationships of these species arediscussed. |
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| Keywords: | Agropyron Elymus RAPDs Thinopyrum Variability |
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