| 家蝇胚胎细胞系染色体制作方法 |
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| 引用本文： | 贺莉芳,赵丹,刘晖,黄学贵,张曦.家蝇胚胎细胞系染色体制作方法[J].畜牧与饲料科学,2011(7):1-2. |
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| 作者姓名： | 贺莉芳 赵丹 刘晖 黄学贵 张曦 |
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| 作者单位： | 遵义医学院寄生虫学教研室,贵州遵义,563003 |
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| 基金项目： | 贵州省科学技术厅基金[黔科合SY(2010)3077号]; 遵义市重大科技项目培育与科技人才培养计划项目[遵科培字(2008)05号] |
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| 摘 要： | 目的]探索家蝇胚胎细胞系(MDEC-07114)染色体制作的方法。方法]以MDEC-07114细胞为材料制备染色体。实验分组:秋水仙素浓度为5、10mg/L组;秋水仙素处理时间为5、6、10h组;预固定组和直接固定组。结果]秋水仙素浓度为10mg/L、作用时间为5h组,细胞分裂相多,染色体形态好;预固定处理后细胞分散好,染色体形态清晰。结论]10mg/L秋水仙素作用5h,经预固定处理后制作MDEC-07114细胞染色体效果最佳。
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| 关 键 词： | 胚胎 细胞系 染色体 家蝇 |
Preparation Methods of the Chromosome of Cell Lines in Musca domestica Embryo |
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| HE Li-fang,ZHAO Dan,LIU Hui,HUANG Xue-gui,ZHANG Xi.Preparation Methods of the Chromosome of Cell Lines in Musca domestica Embryo[J].Animal Husbandry and Feed Science,2011(7):1-2. |
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| Authors: | HE Li-fang ZHAO Dan LIU Hui HUANG Xue-gui ZHANG Xi |
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| Institution: | HE Li-fang,ZHAO Dan,LIU Hui,HUANG Xue-gui,ZHANG Xi (Staff Room of Parasitology,Zunyi Medical College,Zunyi 563003,China) |
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| Abstract: | Objective] The research aimed to explore the preparation method of chromosome of cell lines(MDEC-07114) in Musca domestica embryo. Method] The chromosome was prepared with MDEC-07114 cells as materials. The experimental groups were set up as follows:groups of 5 and 10 mg/L colchicine;groups of treating with colchicine for 5,6 and 10 h,pre-fixative group and the fixed group. Result] More cell division and good chromosome morphology can be observed in the group of treating with 10 mg/L colchicine for 5 h. ... |
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| Keywords: | embryo cell line chromosome Musca domestica |
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