| 陆地棉转激素基因材料的SSR标记分析 |
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| 引用本文： | 潘兆娥,杜雄明,孙君灵,周忠丽,庞保印.陆地棉转激素基因材料的SSR标记分析[J].分子植物育种,2004,2(4):513-519. |
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| 作者姓名： | 潘兆娥 杜雄明 孙君灵 周忠丽 庞保印 |
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| 作者单位： | 中国农科院棉花研究所,农业部棉花遗传改良重点实验室,安阳,455000 |
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| 基金项目： | 本项目由国家高技术研究发展计划(863计划)资助项目和"十五"国家重点科技攻关计划项目(2001BA511B05)资助. |
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| 摘 要： | 利用SSR标记对陆地棉遗传标准系TM-1及其转激素基因后代材料进行。DNA指纹分析。从25对引物中筛选出6对扩增条带差异明显、信号强、背景清晰的引物,选用这6对多态性SSR引物扩增出18个差异片段,其中13个片段呈多态性,占总扩增片段的72．2％。依据扩增结果对42份转激素基因后代材料进行了遗传相似系数分析,构建了分子聚类图,并对其中部分转激素基因材料绘制了SSR指纹图谱。结果表明13个转激素基因材料与TM-1的遗传相似系数都在0．5以下,对这些材料的遗传关系做了初步探讨。
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| 关 键 词： | 陆地棉 转激素基因材料 SSR标记 遗传多样性 |
SSR Marker Analysis of Upland Cotton Transferred into Hormone Gene |
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| Pan Zhaoe Du Xiongming Sun Junling Zhou Zhongli Pang BaoyinCotton Research Institute,Chinese Academy of Agricultural Sciense,Key Laboratory of Cotton Genetic Improvement of Ministry of Agriculture,Anyang.SSR Marker Analysis of Upland Cotton Transferred into Hormone Gene[J].Molecular Plant Breeding,2004,2(4):513-519. |
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| Authors: | Pan Zhaoe Du Xiongming Sun Junling Zhou Zhongli Pang BaoyinCotton Research Institute Chinese Academy of Agricultural Sciense Key Laboratory of Cotton Genetic Improvement of Ministry of Agriculture Anyang |
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| Institution: | Pan Zhaoe Du Xiongming* Sun Junling Zhou Zhongli Pang BaoyinCotton Research Institute,Chinese Academy of Agricultural Sciense,Key Laboratory of Cotton Genetic Improvement of Ministry of Agriculture,Anyang,455000 |
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| Abstract: | Simple sequence repeat (SSR) was used to determine the DNA genetic relationships in genetic marker line of upland cotton (Gossypium hirsutum L.) TM-1 and their variation lines with the transferred hormone genes. 6 pairs of SSR primers were selected to determine the genetic diversity and genetic relationship from 25 pairs of primers. Total 18 alleles were found in 6 pairs of primers, and 13 loci of which were polymorphic, occupied 72.2% of total. Genetic similarity was analyzed for 42 progenies transferred with hormone genes, and a tree diagram and the SSR fingerprint was constructed based on the SSR markers. The result indicated that the similarity coefficient between 13 lines transfered with hormone gene and TM-1 was below 0.5, and the genetic relationships were discussed. |
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| Keywords: | Upland cotton Transgene Genetic diversity SSR marker |
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