| 马铃薯脱毒试管苗壮苗培养体系的优化 |
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| 引用本文： | 赵光磊,吴凌娟,张雅奎,李功义,徐学谱,梁杰,刁琢,董传民,石立航,吕文河.马铃薯脱毒试管苗壮苗培养体系的优化[J].中国马铃薯,2012(4):199-205. |
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| 作者姓名： | 赵光磊 吴凌娟 张雅奎 李功义 徐学谱 梁杰 刁琢 董传民 石立航 吕文河 |
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| 作者单位： | 1. 黑龙江省大兴安岭地区农林科学院, 黑龙江 加格达奇 165000
2. 东北农业大学, 黑龙江 哈尔滨 150030 |
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| 基金项目： | 现代农业产业技术体系建设专项(Zhsyz-1);农业部公益性行业科研专项(3-6-1-6) |
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| 摘 要： | 试验采用正交实验设计法,研究了不同浓度的α-萘乙酸(NAA)、比久(B)9)、6-苄氨基腺嘌呤(6-BA)和大量元素组合对马铃薯品种费乌瑞它脱毒试管苗壮苗培养的影响,以期为费乌瑞它脱毒试管苗壮苗培养优化培养体系的建立提供理论参考。结果表明:在4个因素中对茎粗、根数、根长和单株干重影响大小依次为:大量元素>B9>NAA>6-BA;而对苗高、有效茎节数、活叶数和单株鲜重影响最大的为大量元素,影响最小的为NAA。新复极差法显著性测定结果显示,除4个处理外,其余处理与对照间有效茎节数、活叶数、茎粗、根长、根数、单株鲜重和干重差异均达到极显著水平。MS(100 mL/L大量元素)+0.03 mg/L NAA+10 mg/L B9+2%白糖+0.6%琼脂培养基除根长外各性状的值均最大,表明它是适合‘费乌瑞它’脱毒试管苗壮苗培养的最佳培养基。
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| 关 键 词： | 马铃薯 试管苗 正交设计 费乌瑞它 |
Optimizing for Vigorous Seedling Culture System for the Virus-free Tube Potato Plantlets |
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| Institution: | ZHAO Guanglei1,WU Lingjuan1,ZHANG Yakui1,LI Gongyi1,XU Xuepu1,LIANG Jie1, DIAO Zhuo1,DONG Chuanmin1,SHI Lihang1,LU Wenhe2(1.Daxinganling Academy of Agriculture and Forestry,Jiagedaqi,Heilongjiang 165000,China; 2.Northeast Agricultural University,Harbin,Heilongjiang 150030,China) |
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| Abstract: | Effects of the various combinations of NAA,B9,6-BA and macro-element on the potato plantlets in vitro of cv.Favorita were studied by using the orthogonal experiment design in this research,which would provide a theoretical reference for the establishment of optimized culture system for strengthening plantlets in vitro.Effect order of the factors on stem diameter,root number,root length and plant dry weight was macro-element>B9>NAA>6-BA.For plantlet height,node number,leaf number and fresh weight,macro-element was most influencing,while NAA least influencing.For node number,leaf number,stem diameter,root length,root number,and fresh anddry weight per plantlet in vitro,all treatments but four were highly significantly different from the control.The medium,MS + NAA 0.03 mg/L + B9 10 mg/L + white sugar 2% + agar 0.6%,except root length,gave the highest value,suggesting that this medium is most suitable for 'Favorita' to produce robust plantlets in vitro. |
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| Keywords: | potato plantlets in vitro orthogonal design Favorita |
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