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刺参吐脏再生的组织学研究
引用本文:郑法新,孙修勤,张进兴.刺参吐脏再生的组织学研究[J].中国水产科学,2006,13(1):134-139.
作者姓名:郑法新  孙修勤  张进兴
作者单位:1. 中国海洋大学,生命科学与技术学部,山东,青岛,266003
2. 国家海洋局,第一海洋研究所,山东,青岛,266061
摘    要:以中国山东地区的养殖刺参(Apostichopus jalxmicus)为研究材料,将体长10~15cm的刺参,通过注射KCI(0.35mol/L)诱导吐脏。采用组织学方法对刺参吐脏和再生过程进行观察,结果表明,刺参吐脏后便开始再生,吐脏后的第2天可观察到呼吸树主干及分支结构。腹部肠系膜在吐脏后逐渐增厚,第5天可观察到肠上皮与肌肉组织,再生出具有功能的新肠管的时间大约为2周。本研究旨为刺参再生的进一步研究以及刺参的养殖生产提供基础理论依据。

关 键 词:刺参  吐脏  再生  组织学
文章编号:1005-8737-(2006)01-0134-06
收稿时间:2005-04-19
修稿时间:2005-09-02

Histological studies on evisceration and regeneration in Aponstichopus japonicus
ZHENG Fa-xin,SUN Xiu-qin,ZHANG Jin-xing.Histological studies on evisceration and regeneration in Aponstichopus japonicus[J].Journal of Fishery Sciences of China,2006,13(1):134-139.
Authors:ZHENG Fa-xin  SUN Xiu-qin  ZHANG Jin-xing
Institution:1. cean University of China, Qingdao 266003, China; 2. The First Institute of Oceanography, State Oceanic Administration, Qing dao 266061, China
Abstract:Specimens of A.japonicus(10-15?cm) were collected in the northeast coast of Shandong province.Evisceration was induced by injecting 2-4?ml of KCl 0.35?mol/L into the coelom.We observed the evisceration and regeneration of A.japonicus by applying the method of histology.The result indicated that the A.japonicus started to regenerate immediately after the evisceration,and the respiratory tree trunk and the branched structure were observed on the 2nd day.The mesenteries gradually thickened after evisceration.The intestines epidermis and the muscular tissues were observed on the 5th day.A.japonicus needs 2 weeks to regenerates the newborn intestines.The results provided the information of A.japonicus regeneration for the further study.Journal of Fishery Sciences of China,2006,13(1):134-139]
Keywords:Apostichopus japonicus  evisceration  regeneration  histology
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