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| 浙恢7954农杆菌介导法遗传转化培养基的优化 | |
| 引用本文： | 邱萍,邵健丰,葛敏,李春寿,陶跃之,王华.浙恢7954农杆菌介导法遗传转化培养基的优化[J].江苏农业学报,2012,28(3):461-466. |
| 作者姓名： | 邱萍 邵健丰 葛敏 李春寿 陶跃之 王华 |
| 作者单位： | 1. 浙江师范大学化学与生命科学学院,浙江金华321004;浙江省农业科学院作物与核技术利用研究所分子育种实验室浙江杭州310021 2. 浙江省农业科学院作物与核技术利用研究所分子育种实验室浙江杭州310021 3. 浙江省农业科学院作物与核技术利用研究所分子育种实验室浙江杭州310021;江苏省农业科学院农业生物技术研究所,省农业生物学重点实验室,江苏南京210014 4. 浙江省农业科学院作物与核技术利用研究所水稻研究室,浙江杭州,310021 |
| 基金项目： | 转基因生物新品种培育重大专项 |
| 摘 要： | 以综合性状优良的籼稻品种浙恢7954的成熟胚为材料,比较了在遗传转化不同阶段培养基成分对愈伤组织生长及植株再生的影响,进行遗传转化体系的优化。结果表明,最优的愈伤组织诱导培养基为Y1、愈伤组织抗性选择培养基为J3、愈伤组织分化培养基为D6,在此条件下,GFP转化率达26.8%。 |
| 关 键 词： | 籼稻 成熟胚 根癌农杆菌 遗传转化 绿色荧光蛋白 |
Optimization of culture media for Agrobacterium tumefaciens-mediated transformation of an elite indica cultivar Zhehui 7954 |
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| QIU Ping , SHAO Jian-feng , GE Min , LI Chun-shou , TAO Yue-zhi , WANG Hua.Optimization of culture media for Agrobacterium tumefaciens-mediated transformation of an elite indica cultivar Zhehui 7954[J].Jiangsu Journal of Agricultural Sciences,2012,28(3):461-466. | |
| Authors: | QIU Ping SHAO Jian-feng GE Min LI Chun-shou TAO Yue-zhi WANG Hua |
| Institution: | 1.College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China;2.Crop Molecular Breeding Laboratory,Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;3.Provincial Key Laboratory of Agrobiolgy,Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;4.Rice Research Laboratory,Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China) |
| Abstract: | Agrobacterium meditated-transformations of most rice indica cultivars are far from routine.In this study,the transformation system for an elite indica cultivar,Zhehui 7954,was developed and optimized.The effects of different ingredients in culture media on the growth of callus generated from mature embryo and plant regeneration were investigated.The results showed that Y1,J3 and D6 media were the optimal choice for callus induction,resistance selection and differentiation,respectively.Under the condition,transformation efficiency expressing green fluorescence protein(GFP) was 26.8%. |
| Keywords: | indica rice mature embryo Agrobacterium tumefaciens genetic transformation green fluorescence protein(GFP) |
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