| 不结球白菜抗芜菁花叶病毒基因的遗传分析及SSR标记 |
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| 引用本文： | 彭海涛,宋小明,侯喜林,李英.不结球白菜抗芜菁花叶病毒基因的遗传分析及SSR标记[J].江苏农业学报,2012,28(2):396-401. |
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| 作者姓名： | 彭海涛 宋小明 侯喜林 李英 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室,农业部南方蔬菜遗传改良重点实验室,江苏南京210095 |
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| 基金项目： | 江苏省农业科技自主创新基金项目，江苏省自然科学基金创新学者攀登项目 |
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| 摘 要： | 为了找到与不结球白菜抗芜菁花叶病毒(TuMV)基因连锁的分子标记,采用群体分离分析法(BSA法)和SSR标记进行了与抗病基因连锁标记的筛选。通过对亲本、F1、BC1和F2群体进行病毒接种,研究了试验材料对TuMV的抗性遗传模型,结果表明:不结球白菜对TuMV的抗性由显性单基因控制。通过SSR引物筛选,得到在双亲间稳定表现多态性的引物54对。用这些引物筛选抗感池,得到1个与芜菁花叶病毒抗病基因连锁的SSR标记Ra3E05,连锁距离为8.7 cM。将该标记测序,得到1条184 bp的序列,根据该序列重新设计引物,将SSR标记转化为序列特异扩展区城(SCAR)标记SCRa2,用F2群体对其验证,带型与原SSR标记表现一致,可用于分子标记辅助育种。
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| 关 键 词： | 不结球白菜 芜菁花叶病毒病 分子标记 |
Inheritance and SSR marker of resistance to turnip mosaic virus in nonheading Chinese cabbage |
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| PENG Hai-tao
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SONG Xiao-ming
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HOU Xi-lin
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LI Ying.Inheritance and SSR marker of resistance to turnip mosaic virus in nonheading Chinese cabbage[J].Jiangsu Journal of Agricultural Sciences,2012,28(2):396-401. |
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| Authors: | PENG Hai-tao SONG Xiao-ming HOU Xi-lin LI Ying |
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| Institution: | (State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | To find out the markers linked to the resistance to turnip mosaic virus(TuMV) in non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino),bulk segregant analysis(BSA) and SSR molecular marker were adopted to screen markers in this study.Through TuMV inoculation onto parents,F1,BC1 and F2,the resistance genetic model was analyzed.Results revealed that the resistance to TuMV was controlled by a single dominant gene.Through primer screening,fifty-four pairs of primers showing steady polymorphism in parents were selected.By screening the resistant and susceptible bulks using these primers,one SSR marker Ra3E05 linked to TuMV-resistant gene with a linkage distance of 8.7 cM was identified.The marker was sequenced and a sequence of 184 bp in size was obtained.Base on this sequence,primers were designed and the SSR marker was converted to sequence characterized amplification region(SCAR) marker SCRa2.The segregation pattern of this SCAR marker was the same as that of the SSR marker,and could be applied in marker-assistant breeding. |
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| Keywords: | non-heading Chinese cabbage turnip mosaic virus molecular marker |
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