| 菜豆普通细菌性疫病抗性基因定位 |
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| 引用本文： | 朱吉风,武晶,王兰芬,朱振东,王述民.菜豆普通细菌性疫病抗性基因定位[J].作物学报,2017,43(1):1-8. |
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| 作者姓名： | 朱吉风 武晶 王兰芬 朱振东 王述民 |
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| 基金项目： | This study was supported by the China Agriculture Research System (CARS-09), the National Key Technology R&D Program of China (2013BAD01B03-18a), and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences. |
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| 摘 要： | 菜豆普通细菌性疫病是世界上危害普通菜豆生产的最严重的病害之一。龙芸豆5号是我国黑龙江省的主栽品种,对菜豆普通细菌性疫病表现出良好的抗性。为定位来源于龙芸豆5号的抗性基因,本研究构建了包含785个单株的F2分离群体。基于该群体构建了一张包含206个SSR标记,总长度1648.42 c M,标记间平均遗传距离8.00 c M的遗传图谱。图谱包含12个连锁群,各连锁群平均长度137.37 c M,连锁群上标记数量3~35个。结合温室表型鉴定结果,采用QTL Ici Mapping v4.0软件的完备区间作图法进行QTL定位和效应估计。接种14 d后在Pv06染色体上检测到一个抗病QTL。该位点位于标记p6s249与p6s183之间,加性效应值为0.44,说明增效基因来源于龙芸豆5号,LOD值为5.93,表型贡献率为4.61%,该抗病QTL的效应值相对较低,将在培育稳定持久的抗菜豆普通细菌性疫病的品种中发挥作用。最后,对抗性基因紧密连锁的11对SSR引物与菜豆普通细菌性疫病抗性的关联分析表明,SSR标记p6s249与菜豆普通细菌性疫病抗性极显著关联(P0.001),该标记可用于抗病分子育种。
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| 关 键 词： | 普通菜豆 普通细菌性疫病 SSR QTL 关联分析 |
| 收稿时间： | 2016-05-04 |
Mapping of Common Bacterial Blight Resistance Gene in Common Bean |
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| ZHU Ji-Feng,WU Jing,WANG Lan-Fen,ZHU Zhen-Dong,WANG Shu-Min.Mapping of Common Bacterial Blight Resistance Gene in Common Bean[J].Acta Agronomica Sinica,2017,43(1):1-8. |
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| Authors: | ZHU Ji-Feng WU Jing WANG Lan-Fen ZHU Zhen-Dong WANG Shu-Min |
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| Institution: | Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Common bacterial blight disease is a serious disease affecting the production of common bean (Phaseolus vulgaris L.) worldwide. The common bean germplasm Longyundou 5 is the main cultivar carrying common bacterial blight resistance genein Heilongjiang province. To study the genetic mechanisms behind this resistance, we constructed 785 F2 plants from Longyundou 5. Linkage analysis was performed on this population by using SSR markers. A linkage map covering 1648.42 cM with an average marker distance of 8.00 cM among 206 SSR markers was constructed. This map contained 12 linkage groups with a mean group length of 137.37 cM and the number of loci ranging from three to thirty-five. Combined with the results of phenotypic evaluation, QTL analysis was performed by the inclusive composite interval mapping method with QTL IciMapping v4.0. A QTL was detected between p6s249 and p6s183 on chromosome Pv06, with an additive effect of 0.44, which means the favourable gene of this locus is from Longyundou 5. The LOD score of the QTL was 5.93 and the total phenotypic variation at 14 days after inoculation was 4.61%. These results showed the effect of the QTL was low, which will play an important role in breeding durable resistant varieties of common bean. Association analysis between 11 SSR primers linked with common bacterial blight resistance and the disease rating showed that SSR marker p6s249 is excellently associated (P<0.001) with common bacterial blight resistance and can be used for marker assisted selection. |
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| Keywords: | Phaseolus vulgaris L Common bacterial blight SSR QTL Association analysis |
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