| 应用多因子正交试验筛选野生七姊妹离体培养条件 |
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| 引用本文： | 余晓丽,陶杰,杜敏华,刘宗才.应用多因子正交试验筛选野生七姊妹离体培养条件[J].西北农业学报,2005,14(4):73-76. |
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| 作者姓名： | 余晓丽 陶杰 杜敏华 刘宗才 |
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| 作者单位： | 1. 南阳师范学院生物系,河南,南阳,473061
2. 南阳市园林绿化管理处,河南,南阳,473056 |
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| 基金项目： | 南阳师范学院2003年应用与科技开发项目(nytc200318)
河南省南阳市2004年科技攻关项目(200404016)。 |
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| 摘 要： | 应用正交设计法研究了植物生长调节剂、硝酸银和活性炭等对七姊妹愈伤组织的诱导、不定芽诱导 和生根培养的影响。结果表明:最佳愈伤组织诱导培养基为MS 2．4-D 0．8mg／L 6-BA 0．2 mg／L 蔗糖 30g／L 琼脂7．0 g／L;不定芽诱导最佳培养基为MS NAA 0．1mg／L CPPU0．8 mg／L AgNO34.0mg／L 蔗糖30g／L 琼脂7．0 g／L,分化率为95％,增殖率为5．5;最佳生根培养基为1／4MS NAA 0．3 mg／L IBA 0．3 mg／L 蔗糖20g／L 琼脂4．0 g／L,生根率为90％。
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| 关 键 词： | 七姊妹 幼叶 正交设计 离体培养 方差分析 |
| 文章编号： | 1004-1389（2005）04-0073-04 |
| 收稿时间： | 2005-01-15 |
| 修稿时间： | 2005-06-17 |
Application of Orthogonal Design in Selecting Culture Condition in Vitro of Rose multiflora f. platyphylla Thory |
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| YU Xiao-li,TAO Jie,DU Min-hua and LIU Zong-cai.Application of Orthogonal Design in Selecting Culture Condition in Vitro of Rose multiflora f. platyphylla Thory[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(4):73-76. |
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| Authors: | YU Xiao-li TAO Jie DU Min-hua and LIU Zong-cai |
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| Institution: | Biology Department of Nanyang Teachers College, Nanyang He''nan 473061, China;Garden Virescence Institution of Nanyang, Nanyang He''nan 473056, China;Biology Department of Nanyang Teachers College, Nanyang He''nan 473061, China;Biology Department of Nanyang Teachers College, Nanyang He''nan 473061, China |
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| Abstract: | Using orthogonalty design L9(34),we studied the effect of plant growth regulators ,AgNO3 and activated carbon etc on the induction of callus , adventition buds induction and the adventition bud rooting. The best medium for inducing callus is MS 2,4-D 0. 8mg/L 6-BA 0. 2 mg/L sucrose30 g/L agar 7. 0 g/L; For adventition Buds, the medium is MS NAA 0. 1mg/L CPPU 0. 8 mg/L AgNO34. 0 mg/L sucrose 30 g/L agar 7. 0 g/L, differentiation rate is 95%,the multiplication rate is 5. 5; As for the induction of rooting the only medium is 1/4MS NAA 0. 3 mg/L IBA 0. 3 mg/L sucrose 20 g/L agar 4. 0 g/L,the rooting rate is |
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| Keywords: | Rose multiflora f plutyphylla Thory Young leaves Orthogonally design Culture in Vitro Analysis of variance |
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