| 肝素浓度对辽宁绒山羊精子体外获能的影响 |
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| 引用本文： | 豆兴堂,宋先忱,张兴会,郭丹.肝素浓度对辽宁绒山羊精子体外获能的影响[J].中国草食动物,2011,31(1):21-23. |
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| 作者姓名： | 豆兴堂 宋先忱 张兴会 郭丹 |
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| 作者单位： | 1. 辽宁省辽宁绒山羊原种场有限公司,辽阳,111000
2. 辽宁省畜牧科学研究院 |
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| 基金项目： | 国家绒毛用羊产业技术体系建设辽阳综合试验站项目 |
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| 摘 要： | 在TALP液中添加不同浓度(25、50、100μg/mL)的肝素对辽宁绒山羊精子进行获能处理,在显微镜下检测处理1、2、3、4、5h后的精子活力,用考马斯亮蓝染色法检测获能处理0.5、1、2、4h后的精子获能状况,探讨肝素浓度对绒山羊精子活力、存活时间、获能率的影响。结果发现,在38.5℃、5%CO2、饱和湿度条件下,精子活力随肝素浓度升高而下降,3 h以后25μg/mL肝素组精子活力显著高于其它肝素组(P<0.05)。添加肝素组获能率显著高于对照组(P<0.05),各肝素组精子获能率差异不显著(P>0.05)。对照组精子存活时间最长,25μg/mL肝素组精子存活时间为10.12 h,显著高于其它两组(P<0.05)。表明25μg/mL肝素处理辽宁绒山羊精子体外获能较为适宜。
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| 关 键 词： | 肝素 辽宁绒山羊 精子 体外获能 |
Effects of Heparin on in vitro Sperm Capacitation in Liaoning Cashmere Goats |
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| Institution: | Dou Xing-tang,Song Xian-chen,Zhang Xing-hui,et al2(Liaoning Province Liaoning Cashmere Goat Stock Farm Co.,Limited,Liaoyang 111000,China) |
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| Abstract: | The effects of different heparin densities(25,50,100 μg/mL)on motility,survival time,capacitation rate of sperm from Liaoning cashmere goats were compared.The results indicated that sperm motility decreased with increasing of the heparin densities in 38.5 ℃,saturated humidity,5% CO2 conditions,and after 3 h the sperm motility in 25 μg/mL heparin media was significant higher than that in other heparin media(P<0.05).The rate of sperm capacitation in heparin media was significant higher than that of the control(P<0.05),but no significant difference among different heparin media(P>0.05).The sperm survival time of the control was the longest,that in the 25 μg/mL heparin media was 10.12 h and significant higher than that in other heparin media(P<0.05).It therefore be concluded that the treatment with 25 μg/mL heparin media was a suitable condition for the in vitro sperm capacitation in Liaoning cashmere goats. |
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| Keywords: | heparin Liaoning cashmere goat sperm in vitro capacitation |
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