| 家蚕脂肪酶基因Bmlipase-1在不同BmNPV抗性水平家蚕品种间的表达差异 |
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| 引用本文： | 张婷婷,夏定国,赵巧玲,裘智勇,唐顺明,沈兴家.家蚕脂肪酶基因Bmlipase-1在不同BmNPV抗性水平家蚕品种间的表达差异[J].蚕业科学,2012(4):665-672. |
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| 作者姓名： | 张婷婷 夏定国 赵巧玲 裘智勇 唐顺明 沈兴家 |
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| 作者单位： | 江苏科技大学;中国农业科学院蚕业研究所,农业部蚕桑遗传改良重点实验室 |
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| 基金项目： | 江苏省科技支撑计划(农业)项目(No.BE2011327-2);“十二五”国家科技支撑计划项目(No.2011BAD33B04);国家自然科学基金项目(No.31072083);国家农业科技成果转化资金项目(No.2010GB2C100154);江苏省农业三项工程项目(No.SX[2011]232) |
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| 摘 要： | 家蚕脂肪酶Bmlipase-1在家蚕中肠组织特异性表达,具有抵抗家蚕核型多角体病毒(BmNPV)感染的活性。以对BmNPV具有不同抗性水平的6个家蚕品种为材料,利用荧光定量PCR(qRT-PCR)技术,检测Bmlipase-1基因在不同家蚕品种5龄期健康幼虫中肠组织的表达水平及感染BmNPV后的5龄期幼虫中肠组织中该基因的表达水平变化,探究该基因表达水平与家蚕对BmNPV的抗性水平的关系。结果表明:不同家蚕品种间Bmlipase-1的表达水平差异显著,抗性较强的品种,其表达水平较高,6个供试品种5龄幼虫中肠组织中的Bmlipase-1表达水平依次为NIL.LVR>P50>CVDAR18>nsd.NIL>892>306,抗性较强品种NIL.LVR 5龄期的平均表达量约为抗性较弱品种306的8.2倍;BmNPV能够诱导Bmlipase-1的表达,但不同品种间该基因的诱导表达差异显著,仍表现为抗性较强的品种该基因的诱导表达水平较高,NIL.LVR经BmNPV感染诱导后Bmlipase-1在5龄期的平均表达量约为抗性较弱品种306平均诱导表达水平的12.4倍。推测Bmlipase-1的表达水平与蚕体自身对BmNPV的抗性水平有一定的关联性。
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| 关 键 词： | 家蚕品种 脂肪酶 Bmlipase-1基因 表达水平 荧光定量PCR 家蚕核型多角体病毒 抵抗性 |
Differential Expression of Bmlipase-1 Gene in Bombyx mori Varieties with Different Resistance Levels Against BmNPV |
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| ZHANG Ting-Ting,XIA Ding-Guo,ZHAO Qiao-Ling,QIU Zhi-Yong,TANG Shun-Ming,SHEN Xing-Jia.Differential Expression of Bmlipase-1 Gene in Bombyx mori Varieties with Different Resistance Levels Against BmNPV[J].Acta Sericologica Sinica,2012(4):665-672. |
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| Authors: | ZHANG Ting-Ting XIA Ding-Guo ZHAO Qiao-Ling QIU Zhi-Yong TANG Shun-Ming SHEN Xing-Jia |
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| Institution: | 1,2(1Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China;2Key Laboratory of Genetic Improvement of Silkworm and Mulberry,Ministry of Agriculture,The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China) |
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| Abstract: | Silkworm(Bombyx mori) lipase 1(Bmlipase-1) is specifically expressed in midgut of silkworm larvae,conferring the larvae with resistance against Bombyx mori nucleopolyhedrovirus(BmNPV) infection.Using 6 silkworm varieties with different resistance levels against BmNPV as materials,the expression levels of Bmlipase-1 in midguts of healthy larvae and BmNPV-infected larvae of the 5th instar from different silkworm varieties were detected to explore the relation-ship between expression level of Bmlipase-1 and resistance level of silkworm to BmNPV by fluorescent quantitative PCR(qRT-PCR) technology.The results showed that the expression level of Bmlipase-1 had significant difference in different silkworm varieties.The expression level was higher in silkworm varieties with stronger viral resistance.Expression levels of Bmlipase-1 of the 6 tested silkworm varieties were in the order of NIL·LVR>P50>CVDAR18>nsd·NIL>892>306.The average expression level of Bmlipase-1 in the 5th instar of the highly resistant variety NIL·LVR was about 8.2 times to that of the lowly resistant variety 306.Expression of Bmlipase-1 gene could be induced by BmNPV infection,and the induced expression levels were significantly different in different silkworm varieties.The induced expression level of Bmlipase-1 was also higher in silkworm varieties with stronger viral resistance.After infection by BmNPV,the average expression amount of Bmlipase-1 in the 5th instar of NIL·LVR was about 12.4 times to that of 306,the variety with the lowest resistance.It is suggest that there is certain relevance between expression level of Bmlipase-1 and the resistance level of silkworm against BmNPV. |
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| Keywords: | Silkworm strain Lipase Bmlipase-1 gene Expression level Fluorescent quantitative PCR Bombyx mori nucleopolyhedrovirus Resistance |
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