| 实验大鼠、小鼠胚胎的缓慢冷冻和玻璃化冷冻的比较 |
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| 引用本文： | 徐平,川野佳代,刘丽均,朱冬琴.实验大鼠、小鼠胚胎的缓慢冷冻和玻璃化冷冻的比较[J].上海交通大学学报(农业科学版),2002,20(4):261-265. |
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| 作者姓名： | 徐平 川野佳代 刘丽均 朱冬琴 |
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| 作者单位： | 1. 中国科学院,上海实验动物中心,上海201615
2. 日本熊本大学,医学部实验动物资源研究开发中心,日本熊本860-0811 |
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| 基金项目： | 国家"九五"科技攻关项目(96-A23-06-05) |
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| 摘 要： | 对3日龄小鼠胚胎和5日龄大鼠胚胎进行了玻璃化低温保存和缓慢冷冻保存的比较研究,从经过超数排卵的小鼠和大鼠中收集到了1897个8－16细胞的胚胎,经筛选后将I级和Ⅱ级胚计1738个（91．62%）分别进行两种低温保存试验,并对其中的881个冷冻胚胎分阶段进行复苏,复苏率分别为65．03％（小鼠69．59％,大鼠60．46％）和68．45％（小鼠72．61％,大鼠64．29％）,两者间无显著性差异（P＞0．05）。在复苏在胚胎中,选择其中的488个胚胎进行移植试验,结果,玻璃化冷冻保存胚胎的移植的受孕率为39．47％（89／222）,缓慢冷冻保存胚胎的移植的受孕率为42．56％（115／266）,两种低温保存方法经方差检验也无显著性差异（P＞0．05）。
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| 关 键 词： | 实验大鼠 小鼠 缓慢冷冻 玻璃化冷冻 程控降温冷冻 胚胎移植 |
| 文章编号： | 1000-193X(2002)04-0261-05 |
| 修稿时间： | 2001年11月20 |
Comparison on the cryopreservation methods in mouse and rat embryos: vitrification method versus controlled slow freezing method |
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| XU Ping,KAWANO Kayo,LIU Li jun,ZHU Dong qin.Comparison on the cryopreservation methods in mouse and rat embryos: vitrification method versus controlled slow freezing method[J].Journal of Shanghai Jiaotong University (Agricultural Science),2002,20(4):261-265. |
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| Authors: | XU Ping KAWANO Kayo LIU Li jun ZHU Dong qin |
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| Institution: | XU Ping 1,KAWANO Kayo 2,LIU Li jun 1,ZHU Dong qin 1 |
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| Abstract: | We designed and conducted a trial to determine the accurate pregnancy rates of day 3 mouse and day 5 rat embryos with vitrification and controlled slow freezing method. Total 1897 embryos containing about 8~16 cell were collected from superovulated mouse and rats, and 1738 blastocysts which were assessed as grade 1 and 2 were randomly assigned to each cryopreservation treatment group, and 881 embryos from these frozen embryos were recovered through different stages. The recovery rate were 65 03%(mouse 69 59%, rat 61 46%)and 68 45%(mouse 72 61%,rat 64 29%) respectively. There was not significant difference between these two methods,488 embryos were selected for transplantation trial during the recovery process of embryo. Standard micro surgical methods were used to transfer a total of 488 cryopreservation embryos. Overall pregnancy rate were 39 47%(89/222) for vitrified embryos and 42 56%(115/266) for slowly frozen embryos respectively. Pregnancy rates were not significantly different (ANOVA, P=0 78 or Chi square analysis, P=0 85). The results indicate that vitrification and controlled slow freezing method can be successfully used in cryopreservation mouse and rat embryos without significant reduction in the pregnancy rate, and this result is consistent with some reports. |
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| Keywords: | rat and mouse controlled slow freezing vitrification embryos transplantation |
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