| 3株不同毒力巴氏杆菌外膜蛋白H基因克隆及序列分析 |
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| 引用本文： | 陈小云,邓永,张敏,康凯.3株不同毒力巴氏杆菌外膜蛋白H基因克隆及序列分析[J].江西农业大学学报,2009,31(1). |
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| 作者姓名： | 陈小云 邓永 张敏 康凯 |
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| 作者单位： | 中国兽医药品监察所,北京,100081 |
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| 基金项目： | 国家自然科技资源平台项目 |
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| 摘 要： | 参照GenBank中巴氏杆菌外膜蛋白H(ompH)基因核苷酸序列,设计了一对特异性引物,通过PCR方法,扩增了3株不同毒力猪源巴氏杆菌CA4-1株、679-230株、3397A株ompH全基因.PCR产物克隆至pMD18T载体,进行序列分析,发现3个菌株ompH基因完整的ORF大小分别为1008 bp、1008 bp和1047 bp,分别编码336、336、349个氨基酸,它们的前20个氨基酸均组成信号肽.与已知的15个Heddleston血清型的核苷酸及其推导的氨基酸序列比较,核苷酸同源性在83.3%～99.8%,氨基酸同源性在79.2%～99.4%.序列分析结果表明,ompH基因在不同血清型之间具有较高的同源性.
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| 关 键 词： | 多杀性巴氏杆菌 ompH基因 序列分析 |
Cloning and Sequencing of Outer Membrane Protein H Gene of 3 Different Pasteurella multocide Speices with Different Virulence |
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| CHEN Xiao-yun,DENG Yong,ZHANG Min,KANG Kai.Cloning and Sequencing of Outer Membrane Protein H Gene of 3 Different Pasteurella multocide Speices with Different Virulence[J].Acta Agriculturae Universitis Jiangxiensis,2009,31(1). |
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| Authors: | CHEN Xiao-yun DENG Yong ZHANG Min KANG Kai |
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| Institution: | CHEN Xiao-yun,DENG Yong,ZHANG Min,KANG Kai(China Institute of Veterinary Drug Control,Beijing 100081,China) |
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| Abstract: | According to the sequence of ompH of Pasteurella multocida published in GenBank,a set of specific primers were designed.Using the primers,full-length ompH gene of 3 different Pasteurella multocide speices(C44-1,679-230,3397A) with different virulence was amplified by PCR.The PCR products were cloned into pMD18-T vector for sequencing.Sequence analysis showed that the complete ORF of ompH gene of 3 species were 1008bp,1008bp and 1047bp,encoding 336,336,349 amino acid respectively.Each predicted primary prote... |
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| Keywords: | Pasteurella multocida ompH gene sequence analysis |
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