| 高致病性PRRS活疫苗(HuN4-F112株)抗体对Ⅱ型不同亚群PRRSV的中和效果 |
| |
| 引用本文： | 孟甄祥,安同庆,陈家锃,宫大庆,冷超粮,刘飞,李登云,彭金美,孙艳,吴玉全,吴江,郭娟娟,杨永倩,童光志,田志军.高致病性PRRS活疫苗(HuN4-F112株)抗体对Ⅱ型不同亚群PRRSV的中和效果[J].中国预防兽医学报,2012(5):401-404. |
| |
| 作者姓名： | 孟甄祥 安同庆 陈家锃 宫大庆 冷超粮 刘飞 李登云 彭金美 孙艳 吴玉全 吴江 郭娟娟 杨永倩 童光志 田志军 |
| |
| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室;中国农业科学院上海兽医研究所 |
| |
| 基金项目： | 国家自然科学基金(30901080);国家高技术研究发展计划(863计划)课题(2011AA10A2 08) |
| |
| 摘 要： | 为研究高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)活疫苗(HuN4-F112株)诱导的抗体对II型不同亚群PRRSV的中和作用,本研究将10头PRRSV抗原、抗体阴性的6周龄仔猪,每头仔猪肌肉注射1头份疫苗(106.0TCID50/mL),每周采血并分离血清,检测该血清对II型PRRSV第1、2、4亚群的代表病毒株勃林格PRRSV活疫苗(VR-2332株)、PRRSV活疫苗(CH-1R株)和HP-PRRSV活疫苗(HuN4-F112株)的中和效价。实验结果显示,仔猪免疫3周后开始产生针对HuN4-F112的中和抗体,11周至22周抗体水平达到高峰,抗体持续至少25周,但只有少数免疫猪在几个时间点的血清对VR-2332和CH-1R疫苗株具有血清交叉中和作用,而且中和效价较低。
|
| 关 键 词： | 猪繁殖与呼吸综合征病毒 中和抗体 HuN4-F112 |
Effect of neutralizing antibody induced by HP-PRRS vaccine HuN4-F112 strain on different subgroups of type Ⅱ PRRSV |
| |
| MENG Zhen-xiang,AN Tong-qing,CHEN Jia-zeng,GONG Da-qing,LENG Chao-liang,LIU Fei,LI Deng-yun,PENG Jin-mei,SUN Yan,WU Yu-quan,WU Jiang,GUO Juan-juan,YANG Yong-qian,TONG Guang-zhi,TIAN Zhi-jun.Effect of neutralizing antibody induced by HP-PRRS vaccine HuN4-F112 strain on different subgroups of type Ⅱ PRRSV[J].Chinese Journal of Preventive Veterinary Medicine,2012(5):401-404. |
| |
| Authors: | MENG Zhen-xiang AN Tong-qing CHEN Jia-zeng GONG Da-qing LENG Chao-liang LIU Fei LI Deng-yun PENG Jin-mei SUN Yan WU Yu-quan WU Jiang GUO Juan-juan YANG Yong-qian TONG Guang-zhi TIAN Zhi-jun |
| |
| Institution: | 1(1.Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Harbin 150001,China;2.Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Shanghai 200241,China) |
| |
| Abstract: | To evaluate the neutralization effect of the antibody induced by highly pathogenic porcine reproductive and respiratory syndrome virus(HP-PRRSV) live vaccine(HuN4-F112 strain) on different subgroups of type II PRRSV,ten PRRSV-free pigs were inoculated intramuscularly with 1 dosage(106.0 TCID50) of HuN4-F112 strain.Blood samples were collected weekly for virus isolations and antibody cross-neutralizing tests with the vaccine strains of VR-2332,CH-1R and HuN4-F112,respectively.Results showed that specific neutralizing antibody against HuN4-F112 appeared 3 weeks post inoculation and lasted at least 25 weeks throughout the experiment.Neutralizing antibody titers were maintained at a high level in serum samples collected from 11 to 22 weeks.However,only a few serum samples were able to neutralize VR-2332 and CH-1R strains in a low virus neutralization titers. |
| |
| Keywords: | PRRSV neutralizing antibody HuN4-F112 |
| 本文献已被 CNKI 等数据库收录！ |
|
