| 黄颡鱼三倍体的诱导 |
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| 引用本文： | 杨彩卿,韩烁.黄颡鱼三倍体的诱导[J].安徽农业科学,2008,36(19). |
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| 作者姓名： | 杨彩卿 韩烁 |
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| 作者单位： | 1. 冀中职业学院,河北定州,073000
2. 河北大学生命科学学院分子与生化专业,河北保定,071000 |
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| 摘 要： | 目的]探讨影响黄颡鱼三倍体诱导率的因素和关键技术。方法]采用6-DMAP抑制受精卵极体的释放,诱导黄颡鱼产生三倍体;按6-DMAP浓度(3004、50和600μmol/L),诱导时机(即精卵混合后的时间分别为10和30 min)和诱导持续时间(101、52、0 min),设计正交实验,寻求诱导黄颡鱼三倍体的最优水平组合。结果]黄颡鱼三倍体诱导明显受6-DMAP的浓度、诱导时机和诱导持续时间3因子的影响,而诱导持续时间的长短将直接影响受精卵的诱导率。诱导黄颡鱼三倍体各因素的最优水平组合为:6-DMAP诱导浓度为450μmol/L,精卵混合10 min,诱导持续时间15 min。结论]在黄颡鱼三倍体诱导中,应尽可能地采取温和的手段催产来获得所需要的精卵,以获得高又稳定的三倍体倍化率。
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| 关 键 词： | 黄颡鱼 三倍体诱导 6-DMAP 受精卵 抑制 |
Triploid Induction in Pelteobagrus fulvidraco |
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| YANG Cai-qin et al.Triploid Induction in Pelteobagrus fulvidraco[J].Journal of Anhui Agricultural Sciences,2008,36(19). |
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| Authors: | YANG Cai-qin |
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| Abstract: | Objective] The study aimed to discuss the factors effecting the triploid induction in the Pelteobagrus fulvidraco and the key techniques of induction. Method] The triploid in P.fulvidraco was induced by inhibiting release of polar body of the zygotes with 6-dimethylaminopurine(6-DMAP).In orthogonal experiment with three levels of 6-DMAP(300,450 and 600 μmol/L),the induced occasion(the time after sperms and eggs were confused) of 10,15 and 30 min,and the induced duration of 10,15 and 20 min,the optimum level combination was sought for inducing the triploid in P.fulvidraco.Result] The induction of triploid in P.fulvidraco was obviously effected by 6-DMAP levels,the induced occasion and the induced duration,and the later could directly affect the induction rate of zygotes.The optimum level combination of inducing factors for triploid in P.fulvidraco was that the induction concn.6-DMAP was 450 μmol/L,the time when sperms and eggs was confused was 10 min and the induced duration was 15 min.Conclusion] In the induction of triploid in P.fulvidraco,it should be as much as possible to use gentle ways to induce spawning for getting the needed batch sperms and eggs,thus to obtain the high and stable triploid percentage. |
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| Keywords: | Pelteobagrus fulvidraco Triploid induce 6-DMAP Zygotes Blocking |
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