| GAPB基因原核表达载体的构建 |
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| 引用本文： | 田霞,代其林,龚元亚,孙英坤,谢琳,杨娟,王劲.GAPB基因原核表达载体的构建[J].西南园艺,2011,5(3):1-4. |
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| 作者姓名： | 田霞 代其林 龚元亚 孙英坤 谢琳 杨娟 王劲 |
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| 作者单位： | 西南科技大学植物生物技术研究中心,四川绵阳,621010 |
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| 基金项目： | 国家转基因生物新品种培育重大专项，国家自然科学基金，教育部新世纪优秀人才支持计划，四川省教育厅科技项目 |
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| 摘 要： | 以拟南芥cDNA为模板,用PCR扩增出GAPB的基因全长,然后将GAPB基因片段连接到PET28a（＋）载体上,构建重组质粒并转化大肠杆菌DH5α,经菌落PCR和酶切鉴定筛选出阳性克隆,测序正确后,再将阳性克隆的质粒转化大肠杆菌BL21（DE3）。结果表明：成功构建了原核表达载体PET28a（＋）-GAPB,为后续的G...
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| 关 键 词： | GAPB 大肠杆菌BL21 PET28a(+) 融合蛋白 |
Construction of Glyceraldehyde-3-phosphate dehydrogenase B subunit gene vector expressing in prokaryotic system |
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| TIAN Xia,DAI QiLin,GONG YuanYa,SUN YingKun,XIE Lin,YANG Juan,WANG Jin.Construction of Glyceraldehyde-3-phosphate dehydrogenase B subunit gene vector expressing in prokaryotic system[J].Southwest Horticulture,2011,5(3):1-4. |
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| Authors: | TIAN Xia DAI QiLin GONG YuanYa SUN YingKun XIE Lin YANG Juan WANG Jin |
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| Institution: | 1Plant Biotechnology Research Center,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China;2Biotechnology Research Institute,the Chinese Academy of Agriculture Science,Beijing 100081,China) |
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| Abstract: | In this study,GAPB DNA fragment was amplified by polymerase chain reaction from Arabidopsis cDNA template.The GAPB DNA fragment was then connected into plasmid PET28a（＋）,and was transformed into E.coli DH5α.The positive clones were screened by colony PCR |
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| Keywords: | GAPB E coli BL21 PET28a（＋） The fusion protein |
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