| 拟南芥AtHEMA1基因的克隆及其表达载体的构建 |
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| 引用本文： | 李潇,李翠萍,陈吉裕,苏承刚,张兴国.拟南芥AtHEMA1基因的克隆及其表达载体的构建[J].西南园艺,2011,5(3):10-12,25. |
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| 作者姓名： | 李潇 李翠萍 陈吉裕 苏承刚 张兴国 |
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| 作者单位： | 南方山地园艺学教育部重点实验室,西南大学园艺园林学院,重庆400715 |
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| 基金项目： | 重庆市科委重大攻关项目 |
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| 摘 要： | 为探究AtHEMA1基因转化植物后对叶绿素合成机制的影响,本实验设计特异引物,以拟南芥基因组DNA为模板,采用PrimStar HS DNA聚合酶扩增AtHEMA1基因。将AtHEMA1基因克隆至载体pVCT2101,获得植物表达载体pVCT2298。
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| 关 键 词： | 拟南芥 AtHEMA1基因 克隆 表达载体 |
Cloning of AtHEMA1 Gene and Construction of Its Plant Expression Vector |
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| LI Xiao,LI CuiPing,CHEN JiYu,SU ChengGang,ZHANG XingGuo.Cloning of AtHEMA1 Gene and Construction of Its Plant Expression Vector[J].Southwest Horticulture,2011,5(3):10-12,25. |
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| Authors: | LI Xiao LI CuiPing CHEN JiYu SU ChengGang ZHANG XingGuo |
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| Institution: | (Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Southwest University,Chongqing 400715,China) |
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| Abstract: | The AtHEMA1 gene was amplified via PCR with specially designed primers,PrimStar HS DNA polymerase and Arabidopsis thaliana gDNA,in order to research its influence on plant chlorophyll synthesis.The gene was inserted into vector pVCT2101 and the plant expr |
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| Keywords: | Arabidopsis thaliana AtHEMA1 Cloning Plant expression vector |
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