| 新城疫血凝抑制浓缩抗原的研制与应用 |
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| 引用本文： | 汪德生,;曹登友,;温贵兰,;王开功.新城疫血凝抑制浓缩抗原的研制与应用[J].贵州畜牧兽医,2014(4):10-12. |
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| 作者姓名： | 汪德生 ;曹登友 ;温贵兰 ;王开功 |
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| 作者单位： | [1]贵州大学动物科学学院,贵州贵阳550025; [2]贵州省德江县畜牧局草地中心,贵州德江565200 |
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| 基金项目： | 贵州省农业委员会兽医科技计划项目（2012-07-02）;贵州省农业科技攻关项目“贵州省主要人畜共患病综合防控技术研究”黔科合NY字[2011]3061号 |
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| 摘 要： | 采用甲醛对新城疫LaSota株病毒进行灭活,利用蔗糖透析法进行浓缩,抗原效价由浓缩前的log26上升至浓缩后的log210,在浓缩液中加入25％的丙三醇制成了新城疫血凝抑制抗原.同时对加入稳定剂——丙三醇的自制抗原进行了定质研究.结果表明：加丙三醇自制抗原,温度越低时稳定性越好、保存期最长.将自制抗原、标准抗原与标准血清、待检血清进行血凝抑制试验对比,表明自制抗原准确性、重复性较好,可用于临床监测.
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| 关 键 词： | 新城疫病毒 浓缩抗原 研制 |
Research and Application of Concentrated Hemagglutination Inhibition Antigen of Newcastle Disease Virus |
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| Institution: | Wang Desheng , Chao Dengyou , Wen Guilan , Wang Kaigong (1. College of Animal Science, Guizhou University, Guiyang Guizhou 550025, China; 2. Grassland Center of Dejiang County Animal Husbandry Bureau, Dejiang Guizhou 565200, China) |
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| Abstract: | The Newcastle disease virus（ LaSota strain） was inactivated by formaldehyde and concentrated through sucrose dialysis method, then the antigen titer increased from log2^6 （ before concentrate） to log2^10 （ after concentrate）, after added 25% glycerol as stabilizer to the concentrate virus the hemagglutination inhibition antigen was successfully manufactured, and the antigen was qualitative analysed. The result showed when the temperatare more lower, the self - made antigen more stable and the retention period last more longer. Compare with the standard antigen through hemagglutination inhibi- tion test used standard serum and clinical serum, we found the self - made antigen have good accuracy and repeatability and can be used to clinical survey. |
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| Keywords: | Newcastle Disease Virus Concentrated Antigen Research |
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