| 影响草莓茎尖快繁因素的分析 |
| |
| 引用本文： | 徐启红,王之成,任平国,张胜,侯凯.影响草莓茎尖快繁因素的分析[J].安徽农业科学,2008,36(22). |
| |
| 作者姓名： | 徐启红 王之成 任平国 张胜 侯凯 |
| |
| 作者单位： | 河南大学生命科学院,河南开封,475001;漯河职业技术学院,河南漯河,462000;河南大学生命科学院,河南开封,475001;漯河职业技术学院,河南漯河,462000;漯河天翼生物T程有限公司,河南漯河,462000 |
| |
| 基金项目： | 河南省教育厅自然科学研究科技攻关项目 |
| |
| 摘 要： | 目的]分析几种影响草莓茎尖快繁的因素。方法]以"童子1号"草莓为试材,对草莓茎尖培养快繁中茎尖取材大小、生长调节剂、碳源等影响因素进行分析。结果]以0.5 mm大小的茎尖接种,脱毒率最高,成活率最理想。诱导"童子1号"草莓茎尖成活与生长的最适培养基、最优增殖培养基均为MS+BA 0.5 mg/L。IBA浓度为800 mg/L时,组培苗瓶外生根不仅有较高的生根率和生根数,而且获得了较壮的生根苗。蔗糖和白砂糖作碳源时,繁殖系数较理想,以白砂糖最为适宜。结论]草莓茎尖快繁应以0.5 mm大小的茎尖为材料,诱导分化培养基、继代增殖培养基均为MS+BA 0.5 mg/L,组培苗瓶外生根蘸取的最佳生长调节剂为800 mg/L IBA。
|
| 关 键 词： | 草莓 茎尖培养 快繁 |
Analysis on Influence of Several Factors on Rapid Propagation of Shoot Tip of Strawberry |
| |
| XU Qi-hong et al.Analysis on Influence of Several Factors on Rapid Propagation of Shoot Tip of Strawberry[J].Journal of Anhui Agricultural Sciences,2008,36(22). |
| |
| Authors: | XU Qi-hong |
| |
| Abstract: | Objective] The study was to analyze the several factors of influenced the rapid propagation of shoot tip of strawberry.Method] With strawberry variety Tongzi 1 as tested material,the factors influencing the size of material,growth regulators,carbon source in rapid propagation of shoot tip of strawberry were analyzed.Result] The virus elimination rate of inoculation with 0.5 mm shoot tip was highest and its survival rate was most ideal.The optimum medium and best multiplication medium of induction shoot tip survival and growth of strawberry Tongzi 1all was MS BA 0.5 mg/L.When the concn.of IBA was 800 mg/L,the in vitro rooting of tissue culture seedling not only had high rooting rate and root number,but also obtained stronger rooting seedlings.With sucrose and sugar as carbon source,the propagation coefficient was ideal and that of sugar was optimum.Conclusion] The rapid propagation of shoot tip of strawberry should use 0.5 mm shoot tip as material.The differentiation and subculture medium was MS BA 0.5 mg/L.The optimum growth regulator for soaking the root of tissue culture seedling was 800 mg/L IBA. |
| |
| Keywords: | Strawberry Shoot tip culture Fast reproduction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
