Cold hardiness and transplant response of Juglans nigra seedlings subjected to alternative storage regimes |
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| Authors: | Douglass F Jacobs Barrett C Wilson Amy L Ross-Davis Anthony S Davis |
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| Institution: | 1. Hardwood Tree Improvement and Regeneration Center, Department of Forestry and Natural Resources, Purdue University, 47907-2061, West Lafayette, IN, USA
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| Abstract: | ? Effects of overwinter storage regimes on seedling cold hardiness and physiological vigor are relatively unexplored, particularly for temperate deciduous forest tree species. ? We evaluated influence of storage duration (0, 66, 119, or 175 d) on electrolyte leakage of stem and root collar tissues following exposure to a series of freeze-test temperatures in black walnut (Juglans nigra L.) seedlings sampled from cold (3 °C) or freezer (?2 °C) storage. Seedlings were subsequently transplanted into a controlled growth chamber environment for two months. ? Regardless of storage temperature, mean LT50 was lowest for seedlings stored for 66 d (≤ ?34 °C) and increased dramatically after 119 d (≥ ?13 °C). ? Root collar tissue had lower LT50 than stem tissue after 119 d for cold-stored seedlings, reflecting importance of evaluative tissue type. Days to bud break shortened with increasing storage duration up to 119 d and stabilized thereafter for both storage regimes. Root growth potential was maximized after 119 d of storage, and subsequently declined for cold-stored seedlings. Height growth increased following storage, regardless of duration. ? To promote stress resistance and transplant growth response, we recommend that black walnut seedlings from this genetic source be outplanted after approximately 66–119 d of storage. |
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